The nucleotides and deduced amino acid sequences of porcine myocilin are shown in
Figure 3A . A comparison of the predicted amino acid sequences of pig, bovine, mouse, rat, monkey, and human myocilin is shown in
Figure 3B . The porcine myocilin is composed of 489 amino acids, which is 14 amino acids shorter at the N terminus and lacks one more amino acid at codon 182 than the human myocilin has. The homology of porcine myocilin to mouse, rat, bovine, monkey, and human was 80%, 79%, 84%, 82%, and 82%, respectively. Porcine myocilin is the smallest myocilin described, and also contained a LZ motif at residues 103-152 (
Fig. 3A , dashed underscore). Two predicted N-glycosylation sites and seven O-glycosylation sites were found (
Fig. 3A , squares and circles, respectively). Phosphorylation sites of 31 serine residues, 8 threonine residues, and 5 tyrosine residue were predicted (
Fig. 3A , bold italic). C-terminal olfactomedin-like domain was more conserved in all species than the N-terminal myosin-like domain.