Semithin sections (0.5 μm) were stained with 1% toluidine blue for light microscopic examination. Ten measurements of each of the anatomic regions specified were taken from the midregion of each section by moving the microscope stage 20 μm for each subsequent measurement.
Choroidal thickness was measured by light microscopy as the distance from the basement membrane of the choriocapillaris to the inner edge of the sclera in the following experimental groups: T = 0 hours (
n = 8 animals, 21 sections); T = 2 hours (
n = 5, 12 sections); T = 8 hours (
n = 5, 8 sections); T = 24 hours (
n = 6, 6 sections); T = 48 hours (
n = 6, 6 sections); T = 72 hours (
n = 6, 7 sections); T = 96 hours (
n = 6, 11 sections); T = 120 hours (
n = 6, 14 sections); and non-deprived fellow eyes from 16 animals taken evenly across the recovery period (37 sections;
Fig. 1 ).
Retinal thickness was measured from the inner limiting membrane to Bruch’s membrane (thus including the RPE) and
RPE thickness was measured as the region from the outer limiting membrane to the basal membrane of the RPE (OLM-BM) containing the subretinal space, in the following experimental groups: T = 0 hours (
n = 8 animals, 8 sections); T = 24 hours (
n = 6, 7 sections); T = 48 hours (
n = 6, 7 sections); T = 72 hours (
n = 6, 10 sections); T = 96 hours (
n = 6, 10 sections); T = 120 hours (
n = 6, 14 sections); and non-deprived fellow eyes (
n = 16, 37 sections).