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Ken-ichi Endo, Takahiro Nakamura, Satoshi Kawasaki, Shigeru Kinoshita; Porcine Corneal Epithelial Cells Consist of High- and Low-Integrin β1–Expressing Populations. Invest. Ophthalmol. Vis. Sci. 2004;45(11):3951-3954. doi: 10.1167/iovs.04-0381.
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purpose. Immunohistochemically, all cells in the porcine corneal epithelium, from the superficial to the basal layer, express integrin β1. Flow cytometric study has shown that they comprise integrin β1 high-expressing (β12+) and low-expressing (β1+) populations. This study was undertaken to determine their proliferation characteristics.
methods. Epithelial cells from porcine corneas were sorted and labeled with anti-integrin β1 antibody and a fluorescent-dye–conjugated secondary antibody. The fluorescent intensity of labeled cells was analyzed and β12+ and β1+ cells were cultured in an adhesive-coated culture plate.
results. Flow cytometry demonstrated that the epithelial cells comprised two distinct populations with a similar ratio throughout the cornea. Whereas β12+ cells attached and grew to confluence in the plate, β1+ attached only transiently to the plate and exhibited minimal growth.
conclusions. The data indicate that the porcine cornea contains two distinct populations of epithelial cells, one exhibiting high and the other low integrin β1 expression. The observation that β12+ cells had greater growth potential suggests that they may represent an enriched population of transit-amplifying cells.
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