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Henry J. Klassen, Tat Fong Ng, Yasuo Kurimoto, Ivan Kirov, Marie Shatos, Peter Coffey, Michael J. Young; Multipotent Retinal Progenitors Express Developmental Markers, Differentiate into Retinal Neurons, and Preserve Light-Mediated Behavior. Invest. Ophthalmol. Vis. Sci. 2004;45(11):4167-4173. doi: 10.1167/iovs.04-0511.
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purpose. To use progenitor cells isolated from the neural retina for transplantation studies in mice with retinal degeneration.
methods. Retinal progenitor cells from postnatal day 1 green fluorescent protein-transgenic mice were isolated and characterized. These cells can be expanded greatly in culture and express markers characteristic of neural progenitor cells and/or retinal development.
results. After they were grafted to the degenerating retina of mature mice, a subset of the retinal progenitor cells developed into mature neurons, including presumptive photoreceptors expressing recoverin, rhodopsin, or cone opsin. In rho−/− hosts, there was rescue of cells in the outer nuclear layer (ONL), along with widespread integration of donor cells into the inner retina, and recipient mice showed improved light-mediated behavior compared with control animals.
conclusions. These findings have implications for the treatment of retinal degeneration, in which neuronal replacement and photoreceptor rescue are major therapeutic goals.
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