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Lu Chen, Claus Cursiefen, Stefano Barabino, Qiang Zhang, M. Reza Dana; Novel Expression and Characterization of Lymphatic Vessel Endothelial Hyaluronate Receptor 1 (LYVE-1) by Conjunctival Cells. Invest. Ophthalmol. Vis. Sci. 2005;46(12):4536-4540. doi: 10.1167/iovs.05-0975.
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purpose. Lymphatic vessel endothelial hyaluronic acid receptor (LYVE-1) is a newly discovered lymphatic-specific marker. To date, there is no report of its expression on conjunctival cells. The purpose of this study was to investigate the expression of LYVE-1 in normal conjunctiva, to phenotype LYVE-1+ cells, and to study changes in their expression levels during corneal inflammation.
methods. Flatmounted conjunctivae or cross sections of eyeballs were harvested from BALB/c mice (6–8 weeks of age) for immunofluorescent confocal microscopic studies.
results. The data demonstrate, for the first time, that in addition to its expression on lymphatic vessels, LYVE-1 was expressed on CD45+, CD11b+, and CD31− conjunctival cells, indicating a bone-marrow–derived monocytic lineage. Surprisingly, the number of cells that expressed LYVE-1 decreased during corneal inflammation, in conjunction with ingrowth of lymphatics into the cornea.
conclusions. A new population of monocytic cells has been found to express LYVE-1 in normal conjunctiva. These cells that normally express LYVE-1 may act as a reservoir for lymphangiogenesis and cell recruitment when the immune system is challenged.
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