To document double- and triple-labeled immunohistochemistry, a confocal laser-scanning microscope (MRC 1000; Bio-Rad, Hercules, CA; attached to a Diaphot 300; Nikon, Düsseldorf, Germany; equipped with a krypton-argon laser; ALC, Salt Lake City, UT; and with 20× dry or 40× and 60× oil-immersion objective lenses, with numeric apertures of 0.75, 1.30, and 1.4, respectively; Nikon) was used. Sections were imaged using the appropriate filter settings for DTxR (568 nm excitation, filter 605DF32; channel 1, coded red), Alexa488 (488 nm excitation, filter 522DF32; channel 2, coded green), and Cy5/Alexa647 (647 nm excitation, filter 680DF32; channel 3, coded blue). Colocalization of signals in channels 1 and 2 resulted in a yellow mixed color. An overlap of signals in channels 1 and 3 resulted in pink, and in channels 2 and 3 in turquoise (mixed) colors. For quantitative assessments, a fluorescence microscope (10×, 20×, or 40× dry objective lenses; model BX50; Olympus, Melville, NY) equipped with a digital camera (model DP70; Olympus) was used.