October 1965
Volume 4, Issue 5
Free
Articles  |   October 1965
Incorporation of sulfate-35S, N-acetylglucosamine-1-14C, glucose-1-14C, and galactose-1-14C into Calf and Beef Corneal Glycosaminoglycans
Author Affiliations
  • CLAES H. DOHLMAN
    Corneal Research Unit, Institute of Biological and Medical Sciences, Retina Foundation, Boston, Mass., and Department of Ophthalmology, Washington University School of Medicine, St. Louis, Mo.
  • BERNARD WORTMAN
    Corneal Research Unit, Institute of Biological and Medical Sciences, Retina Foundation, Boston, Mass., and Department of Ophthalmology, Washington University School of Medicine, St. Louis, Mo.
  • SIW HULTMAN
    Corneal Research Unit, Institute of Biological and Medical Sciences, Retina Foundation, Boston, Mass., and Department of Ophthalmology, Washington University School of Medicine, St. Louis, Mo.
Investigative Ophthalmology & Visual Science October 1965, Vol.4, 867-871. doi:https://doi.org/
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      CLAES H. DOHLMAN, BERNARD WORTMAN, SIW HULTMAN; Incorporation of sulfate-35S, N-acetylglucosamine-1-14C, glucose-1-14C, and galactose-1-14C into Calf and Beef Corneal Glycosaminoglycans. Invest. Ophthalmol. Vis. Sci. 1965;4(5):867-871. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Beef corneas were incubated in vitro with N-acetylglucosamine-1-14C, glucose-1-14C, and galactose-1-14C. Calf and beef corneas were incubated in vitro with inorganic sulfate-35S. Corneal glycosaminoglycans were isolated and the mixture resolved by use of ECTEOLA anionexchanger and further fractionation with ethanol. After incubation ivith glucose-1-14C, a higher specific activity was found in keratan sulfate than in chondroitin-chondroitin sulfate, whereas the opposite was found after incubation with galactose-1-14C and sulfate-35S. Incubation of corneas with N-acetylglucosamine-1-14C resulted in approximately the same specific activity in keratan sulfate and chondroitin-chondroitin sulfate. The results are discussed in terms of the normal biosynthesis of corneal glycosaminoglycans.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×