Immunohistochemical localization of D-β-Asp–containing peptides. Examples of the immunohistochemical localization of the D-β-Asp–containing peptides in a fetus (36 weeks; A, D, G, J, M, P) and a 74-year-old man (B, E, H, K, N, Q) are shown. In the eyes of fetuses or of donors younger than 18 years, no immunoreactivity to the D-β-Asp–containing peptides was seen in the lens (A), ciliary body (D), retina, choroid, sclera (G, J, M), or optic nerve head (P). In contrast, in the eyes of elderly donors, relatively strong immunoreactivity to the D-β-Asp–containing peptides was observed in the nuclei of the lens (B, arrowhead), nonpigmented ciliary epithelial cells (E), sclera (H, arrow), and drusen (N, Q, arrowhead). In addition, moderate immunoreactivity to the D-β-Asp–containing peptides was found in the cortex of the lens (B, arrow), internal limiting membrane (K, arrow), thickened Bruch membrane, blood vessels in the retina (N, arrows), and lamina cribrosa (Q, arrows). The addition of an excess peptide containing D-β-Asp resulted in reduction of immunoreactivity (C, F, I, L, O, R). Scale bars: (A–C, G–L, P–R) 100 μm; (D–F, M–O) 20 μm..