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Wei Chi, Xuefei Zhu, Peizeng Yang, Xiaoli Liu, Xiaomin Lin, Hongyan Zhou, Xiangkun Huang, Aize Kijlstra; Upregulated IL-23 and IL-17 in Behçet Patients with Active Uveitis. Invest. Ophthalmol. Vis. Sci. 2008;49(7):3058-3064. doi: 10.1167/iovs.07-1390.
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purpose. Behçet disease (BD) is a systemic inflammatory disease presumably caused by an autoimmune response. The interleukin (IL)-23/IL-17 pathway has been demonstrated to be involved in the development and maintenance of certain inflammatory diseases. This study was designed to investigate the role of IL-23 and IL-17 in BD.
methods. IL-23p19 mRNA in peripheral blood mononuclear cells (PBMCs) was examined using RT-PCR. The levels of IL-23, IL-17, and IFN-γ in sera or PBMCs were detected by ELISA. Flow cytometry was used to evaluate the frequencies of IL-17–producing and IFN-γ–producing T cells and the expression of CD45RO.
results. Results showed that the expression of IL-23p19 mRNA, IL-23, IL-17, and IFN-γ was markedly elevated in BD patients with active uveitis. The frequencies of IL-17–producing and IFN-γ–producing T cells from PBMCs were significantly upregulated in BD patients with active uveitis. The increased IL-17 (3.10% ± 0.53%) in BD patients with active uveitis was primarily produced by CD45RO+ memory T cells. Recombinant (r) IL-23 could upregulate IL-17 production by polyclonally stimulated PBMCs, whereas interferon (IFN)-γ downregulated IL-17 production.
conclusions. These findings reveal that the levels of IL-23, IL-17, and IFN-γ are elevated in BD patients with active uveitis, and they suggest that the IL-23/IL-17 pathway together with IFN-γ is associated with the active intraocular inflammation in BD patients.
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