Investigative Ophthalmology & Visual Science Cover Image for Volume 47, Issue 8
August 2006
Volume 47, Issue 8
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Retinal Cell Biology  |   August 2006
Potential Role of Microglia in Retinal Blood Vessel Formation
Author Affiliations
  • Daniella Checchin
    From the Departments of Pediatrics, Ophthalmology and Pharmacology, Research Center, Hôpital Ste. Justine, Montreal, Quebec, Canada;
    Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada;
  • Florian Sennlaub
    From the Departments of Pediatrics, Ophthalmology and Pharmacology, Research Center, Hôpital Ste. Justine, Montreal, Quebec, Canada;
    INSERM U598, Physiopathologie des Maladies Oculaires: Innovations Therapeutiques, Paris, France; and
  • Etienne Levavasseur
    INSERM U712, Université Pierre et Marie Curie, Hôpital St. Antoine, Paris, France.
  • Martin Leduc
    From the Departments of Pediatrics, Ophthalmology and Pharmacology, Research Center, Hôpital Ste. Justine, Montreal, Quebec, Canada;
  • Sylvain Chemtob
    From the Departments of Pediatrics, Ophthalmology and Pharmacology, Research Center, Hôpital Ste. Justine, Montreal, Quebec, Canada;
    Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada;
Investigative Ophthalmology & Visual Science August 2006, Vol.47, 3595-3602. doi:https://doi.org/10.1167/iovs.05-1522
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      Daniella Checchin, Florian Sennlaub, Etienne Levavasseur, Martin Leduc, Sylvain Chemtob; Potential Role of Microglia in Retinal Blood Vessel Formation. Invest. Ophthalmol. Vis. Sci. 2006;47(8):3595-3602. https://doi.org/10.1167/iovs.05-1522.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

purpose. The role of microglia, present in the retina early in development before vascularization, remains ill defined. The authors investigated whether microglia are implicated in retinal blood vessel formation.

methods. The microglia and vasculature of developing human fetal and rodent retinas were examined by labeling the endothelial cells with lectin and the microglia with CD18 antibody or green fluorescent protein driven by the promoter of the chemokine receptor CX3CR1. Rodent ischemic proliferative retinopathy induced by hyperoxia or hypercapnia, which model retinopathy of prematurity, and ex vivo retinal explants were used to assess microglial involvement in vascular pathology. Microglial participation in developmental retinal vessel formation was further studied in neonatal rats after pharmacologic macrophage depletion with the use of clodronate liposomes and subsequent intravitreal injection of microglia.

results. Microglia intimately appose developing vessels of human and murine retinas. Ischemic retinopathy models exhibit decreased microglia concomitant with the characteristic reductions in vasculature observed in these retinopathies. Retinal explants exposed to conditions resulting in ischemic retinopathies (in vivo) reveal that antioxidants protect against microglial loss. Depletion of resident retinal microglia, but not systemic macrophages, reduced developmental vessel growth and density, which were restored by intravitreal microglial injection.

conclusions. These observations suggest that proper retinal blood vessel formation requires an adequate resident microglial population because diminished microglia are associated with decreased vascularity in models of ischemic retinopathy and retinal vascular development. In light of these findings, the traditional definition of microglia as merely immunocompetent cells should be reconsidered to encompass this new function related to blood vessel formation.

Vascularization of the retina is a complex process involving astrocytes and endothelial cells (ECs) stimulated by the increasing oxygen (O2) demands of differentiating neural cells. 1 Astrocytes enter the retina through the optic nerve 2 and distribute ahead of the growing vascular front, 3 forming a physical template on which ECs organize their vascular network. 4 Specialized ECs, termed tip cells, are located at the ends of the vascular sprouts and guide blood vessel growth through the tissue, whereas ECs of the blood vessel stalk proliferate to form patent capillaries. 5 In ischemic proliferative retinopathies (e.g., retinopathy of prematurity [ROP] and diabetic retinopathy), this organized retinal vessel formation is interrupted, and reductions in vascularity are followed by abnormal intravitreal neovascularization. However, the mechanisms necessary for proper intraretinal blood vessel formation and the factors in the ischemic retina that inhibit this process under pathologic conditions are not clearly understood. 
Retinal microglia comprise a heterogeneous population of cells, believed to be of hemangioblastic mesodermal origin, 6 all which constitutively express CD45 (leukocyte common antigen) and major histocompatibility complex (MHC)-I and II, with a subpopulation also bearing macrophage-specific markers. 7 8 As such, they are considered specialized local immunocompetent cells. During human retinal development, microglia lacking macrophage markers are present by 10 weeks of gestation (WG), before astrocyte invasion and onset of vasculogenesis, whereas those positive for macrophage antigen (S22) appear at approximately 14 WG, the beginning of vascularization. 9 It has been suggested that this latter subpopulation represents vessel-associated perivascular microglia detected in the adult retina 8 10 ; along these lines, microglia have been shown to extend their processes to orientate toward and contact blood vessels as they form. 11 12 13 However, the functional inference of these observations remains speculative. 
Since the late 1970s, the macrophage has been recognized as a key angiogenic effector cell. 14 15 16 17 18 Macrophages induce angiogenesis by secreting factors that are pro-angiogenic, break down basement membrane, and stimulate other cells to produce greater amounts of pro-angiogenic substances. 14 In this context, microglia are also known to release a number of pro-angiogenic products, including growth factors (e.g., transforming growth factor-β, 19 basic fibroblast growth factor 20 21 ), matrix metalloproteinases, 22 23 and cytokines (e.g., tumor necrosis factor [TNF]-α, interleukin-1 24 25 ). Thus, although it is feasible that microglia, as resident central nervous system macrophages, may play roles similar to those of macrophages in blood vessel formation, little consideration has been given to such a possibility. We therefore hypothesized that microglia are involved in the formation of retinal blood vessels. 
Herein we illustrate that microglial deficit leads to diminished blood vessel formation in the retina. The loss of microglia in two different models of ROP was shown to occur concomitantly with the phase characteristic of vascular reduction. In addition, in developmental retinal vascularization, pharmacologic depletion of resident microglia was also associated with decreased vascularity, an effect that intravitreal injection of microglia corrected. Together these observations highlight a novel function of microglia, broadening its designation as a specialized immunocompetent cell by disclosing its role in retinal blood vessel formation. 
Materials and Methods
Human Samples
Fetal eyes were collected from terminated pregnancies after the receipt of informed, signed, parental consent according to guidelines approved by the Ethics Committee of Hôpital Ste. Justine. Subjects with ocular malformations or medical conditions related to vascular impairment were excluded. 
Animals and Models of Retinopathy of Prematurity
The fractalkine (CX3CL1) receptor CX3CR1 is present on retinal microglia. 26 As such, eyes from postnatal day (P) 6 mice expressing green fluorescent protein (GFP) under the CX3CR1 promoter (CX3CR1GFP/+) on a C57BL/6 background, as previously described, 27 were used to examine microglia with respect to developing retinal blood vessels. 
A model of O2-induced retinopathy 28 was used with slight modifications. C57BL/6 mice (Charles River, St. Constant, QC, Canada) were exposed to 75% O2 (Oxycycler A820CV; BioSpherix Ltd., Redfield, NY) from P7 to P12, producing a loss of central retinal microvasculature. 
Elevated arterial carbon dioxide (CO2) has been identified as a risk factor for ROP. 29 30 A protocol of raised inspired CO2 level, previously shown to retard normal retinal vascular development, 30 31 was followed with minor changes. Sprague-Dawley rats (Charles River) were exposed to 10% CO2 within 24 hours of birth for 12 hours, a period sufficient to significantly reduce the vascularized area of the retina. 31  
Sprague-Dawley rats were used for all retinal explant cultures, clodronate liposome experiments, and cerebral microglial isolations, and Yorkshire piglets (Fermes Ménard, L’Ange-Gardien, QC, Canada) were used to derive retinal microvascular ECs. 
All procedures were approved by the Animal Care Committee of Hôpital Ste. Justine and conformed with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research. 
Retinal Wholemounts
Eyes were fixed in 4% paraformaldehyde. Retinas were dissected free, subjected to 100% methanol (−20°C) for 10 minutes, and incubated overnight (room temperature) in 1% Triton X-100/phosphate-buffered saline (PBS) with either the TRITC-conjugated lectin EC marker Ulex europaeus (1:100; Sigma-Aldrich, St. Louis, MO) and an antibody against the microglial marker CD18 (1:50; PharMingen, BD Biosciences, Mississauga, ON, Canada) (human samples) or the tetramethylrhodamine isothiocyanate (TRITC)–conjugated lectin EC/microglial marker Griffonia simplicifolia 11 (rodent samples) (1:100; Sigma-Aldrich). Double-labeled retinas were incubated with a monoclonal Alexa–conjugated secondary antibody (1:10 000; Molecular Probes, Burlington, ON, Canada) for 1 hour (room temperature) before all retinas were washed in PBS and mounted. Negative control experiments were performed in parallel by incubating retinas in 1% Triton X-100/PBS alone, followed by secondary antibody if one was used. 
Retinal Explants
Retinal explant cultures were established as we and others have previously reported. 32 33 34 In short, P6 rats were euthanatized, eyes were enucleated, and retinas were immediately dissected free under aseptic conditions. Each retina was placed in a drop of 1% fetal bovine serum (FBS)/DMEM (Nuclepore Track-Etch Membrane; Whatman, Florham Park, NJ) resting on the surface of 6 mL of 1% FBS/DMEM in a six-well plate. The explants were cultured in a humidified 37°C atmosphere under standard (5% CO2/21% O2), high O2 (5% CO2/75% O2), or high CO2 (10% CO2/21% O2) culture conditions. The peroxynitrite decomposition catalyst 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrinato iron (III), chloride (FeTPPS; Calbiochem, Mississauga, ON, Canada) and the superoxide dismutase (SOD) mimetic copper[II] [3,5-diisopropylsalicylate acid]2 (CuDIPS; Calbiochem) were added to the media at experiment onset (final concentration, 10 μM). After 3 days, the explants (drug treated and controls) were fixed in 4% paraformaldehyde and prepared as described here with the general microglial marker OX-42 (1:50; Serotec, Raleigh, NC) followed by Alexa-conjugated monoclonal secondary antibody. 
Clodronate Liposome Preparation
Clodronate liposomes elicit selective depletion of macrophages by apoptosis 35 36 and have been studied in systems demonstrating their insensitivity toward other cell types. 36 37 38 Thus, liposomes with entrapped clodronate were produced as detailed. 39 Briefly, 86 mg phosphatidylcholine (Sigma-Aldrich) and 8 mg cholesterol (Sigma-Aldrich) were dissolved in chloroform. After low vacuum rotary evaporation (37°C) of the chloroform phase, the lipid film was dispersed in 10 mL of a 0.6 M clodronic acid solution. After swelling, nonencapsulated clodronate was removed by centrifugation, and clodronate-containing liposomes were washed and resuspended in 4 mL sterile PBS. 
Cell Isolation and Cell Death Assay
To ensure that clodronate liposomes do not affect EC viability, the effects of clodronate liposomes on EC survival were tested on cultured ECs. To obtain sufficient yields, primary ECs were derived from porcine retinal microvessels. 40 Microglia were isolated from early postnatal rat brain as previously described. 41 After 24-hour exposure to clodronate liposomes (5 μL), MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide; Sigma-Aldrich; 0.5 mg/mL in PBS) was incubated with cells for 2 hours (37°C). Media were then aspirated, the formazan product produced through the reduction of MTT by viable cells was solubilized with acidified isopropanol, and optical density was measured at 600 nm. 
In Vivo Macrophage Depletion with Clodronate Liposomes and Intravitreal Injection of Microglia
Within 24 hours of birth (i.e., at the approximate onset of retinal vascularization), rats were injected intraperitoneally (60 μL) and intravitreally (4 μL; FemtoJet Microinjector; Eppendorf, Toronto, ON, Canada) with clodronate liposomes except where otherwise stated. Because liposomes cannot cross the blood/brain-retinal barrier, 42 intravitreal injection was necessary to deplete resident macrophages (i.e., retinal microglia). Administration of PBS alone, not liposomes containing PBS, is considered the proper control because comparisons should be made to animals with normal nonblocked, nonsuppressed, and nonactivated macrophages. 39 Rats were euthanatized and their eyes were enucleated 5, 6, and 8 days after clodronate liposome treatment. Eyes were processed for retinal wholemounting, as described here, or paraffin embedding and sectioning. The sections (7 μm) were cut sagittally, parallel to the optic nerve, and processed for periodic acid-Schiff and hematoxylin staining. 
To add back microglia, on the day of the experiment microglial cells were trypsinized, counted, and resuspended in media to yield 105 cells/intravitreal injection (FemtoJet Microinjector; Eppendorf). Six days after clodronate liposome treatment (at P6), rats were injected intravitreally with media alone or microglia. At P10, all animals were euthanatized and retinal wholemounts were prepared, as described here. 
Quantification Methods
Images were captured (Eclipse E800 microscope; Nikon, Tokyo, Japan); individual images were captured in the same plane. All measurements were made by two masked observers (Image-Pro Plus software, version 4.1; Media Cybernetic, Silver Spring, MD). Microglia of the superficial layer were counted from three randomly selected 40× fields of view per retina. The surface area of the retina covered by vessels was measured and expressed as a percentage of the entire retinal area to obtain the vascularized area. Vessel density was determined by tracing all vessels present in three randomly selected vascularized areas and dividing the total length (in μm) by the total area (in μm2). For uniformity, all vascularity measurements are presented as a percentage of the control. 
Statistical Analysis
Data were analyzed, as required, by Student t test or one-way ANOVA with comparisons among the means performed by the appropriate post hoc test. Statistical significance was set at P < 0.05. 
Results
Microglia Ahead, and in Intimate Apposition, of Retinal Blood Vessels
In humans of 15 WG, retinal blood vessels are just beginning to emanate from the optic disc, whereas microglia already occupy the entire retinal surface (Figs. 1A 1B 1C) . With the use of transgenic mice that expressed GFP specifically in microglial cells, we were able to visualize a similar topographic relationship because at P6 the microglial cells were present beyond the vascular front (Figs. 1E 1F 1G) . Moreover, the GFP-positive microglia revealed, with unprecedented precision, the apposition of their processes with the endothelial tip cell filopodia and endothelial stalk cells (Fig. 1H) . Throughout the developing retina, including the tip and stalk cells of the vascular front, all ECs appeared to be in intimate proximity of microglia (Figs. 1D 1H and 1I)
Ischemic Retinopathy Models of Retinopathy of Prematurity Associated with Loss of Microglia
Models of ROP have been reproduced in animals exposed to hyperoxia and hypercapnia (augmented arterial CO2) 28 30 ; these factors elicit oxidative and nitrative stress, respectively, and contribute to ischemic proliferative retinopathies. 31 43 44 We determined the impact of such conditions on microglia in corresponding models of ROP. Retinal wholemounts from mice exposed to hyperoxia have a marked loss of microglia in the areas coinciding with central capillary dropout characteristic of this model (Fig. 2A) ; microglia were sparsely present in the remaining vascularized regions. Similarly, rats subjected to CO2 exhibited significantly reduced microglial counts (Fig. 2B)when hypercapnia was shown to retard vessel growth. 31 It should be noted that because these rats were 36 hours old or younger, the extent of retinal vascularization was minimal (approximately 18% of surface); however attenuation of vascular development was clearly detected in these and in older animals. 30 31 Thus, an early or a late loss of microglia was associated with a coincidental decrease in retinal vascularity. 
Free Radical Mediation of Microglial Loss
To corroborate the in vivo microglial loss and to elucidate a potential mechanism underlying this observation, ex vivo retinal explants were exposed to hyperoxic and hypercapnic conditions in the presence and absence of antioxidants (Fig. 3) . The SOD mimetic CuDIPS was used to combat the reactive O2 species superoxide in the O2-exposed group, whereas the peroxynitrite decomposition catalyst FeTPPS was selected to minimize (protein) nitration observed in retinas exposed to high levels of CO2. 31 A difference in microglia morphology was noted between hyperoxia- and hypercapnia-exposed tissues that might have been caused by cell activation or impending cell death (especially in the more severe hyperoxia-exposed tissues). Regardless, both antioxidants protected against microglial loss (Fig. 3) , as reported for the vasculature. 31 44 Because of the close relationship between microglia and ECs, we could not establish the precise sequence of cell types affected in these retinopathy models; therefore, we proceeded to determine the effect of microglial depletion on developmental retinal blood vessel formation. 
Resident Macrophages Necessary for Normal Retinal Vascular Development
The impact of microglia on developmental retinal blood vessel formation was studied in neonatal rats undergoing retinal vascularization by depleting and subsequently intravitreally injecting retinas with microglia. Microglia were specifically diminished by clodronate liposomes (Figs. 4A 4B) , which had no negative effect on EC viability (in culture) (Fig. 4B) . With the exception of the diminished presence of EC nuclei along the inner surface of the retina, the retinal structure was unaltered by the clodronate liposome treatment (Fig. 4C)
Five days after pharmacologic depletion of systemic macrophages and resident retinal microglia, pronounced decreases in retinal vascular area and density were observed (Figs. 5A 5B) . This effect persisted until at least 8 days after treatment, at which point there was a slight tendency toward recovery of vascular density (Figs. 5A 5B) . In addition, 8 days after treatment, the microglial count no longer significantly differed from that of the vehicle (Fig. 4A)
We distinguished the role of systemic macrophages from that of resident retinal microglia by administering clodronate liposomes either intraperitoneally or intravitreally. The retinal vascularity of rats receiving both injections was comparable to those receiving intravitreal injections alone (Fig. 6A) . Similarly, retinal vascularity was indistinguishable between animals injected intraperitoneally with clodronate liposomes or PBS (Fig. 6A) ; intraperitoneal clodronate liposomes diminished systemic macrophages by approximately 70%, 45 indicating a prominent role for resident retinal microglia, as opposed to systemic macrophages, in normal retinal blood vessel formation. Moreover, intravitreal injection of microglia into microglia-depleted retinas rescued the diminished vascularity observed in clodronate-treated rats (Fig. 6B)
Discussion
The contribution of macrophages to blood vessel formation is well recognized. 14 46 47 Although most studies focus on their angiogenic involvement in tumor development, wound healing, and inflammation, macrophages reportedly play a similar role in choroidal abnormalities 48 49 and in normal retinal development. 50 However, the participation of microglia in retinal blood vessel formation has received little attention to date. This study reveals that ischemic proliferative retinopathy is associated with a loss of resident microglia and that developmental retinal blood vessel growth and density are altered by specific pharmacologic microglial depletion (and subsequent intravitreal injection of microglia). 
Microglia of the fetal and adult human retina are restricted to the inner vascularized regions (i.e., the nerve fiber/ganglion cell layer and the inner and outer plexiform layers), 8 51 appearing first before vascularization and then later along with the onset of vasculogenesis. 51 Yet the reason for their presence remains speculative. It has been suggested that microglia are attracted to the retina to phagocytose the pyknotic debris that accompanies neural remodeling. 52 However, this process occurs through centrifugal orientation, whereas, on the contrary, microglia appear in greatest number at the periphery as the developing human retina becomes more abundant centrally throughout gestation. 51 Furthermore, because microglial invasion significantly precedes neuronal death, 11 51 microglial numbers increase beyond the peak of pyknosis, 51 and adjacent retinal cells—not macrophages—are largely responsible for engulfing most pyknotic cells, 53 it seems likely that microglia have other functions in retinal development. 
In rat neural allografts, transplantation of neural cell suspensions into intact striatum reveals that microglia appear before blood vessels, after which they are in proximity with the vascular sprouts and become intimately associated with the ingrowing vasculature. 13 Our observations are consistent with these neural system findings: (1) The retina is populated with microglia before vascular development in rodents and humans (Figs. 1 2) . (2) Models of ischemic retinopathy induced by hyperoxia and hypercapnia reveal a loss of microglia that coincides with vasoattenuation (Figs. 2 3) . These retinal vasculopathy-inducing conditions 31 43 44 also evoke free radical–mediated damage against microglia (Fig. 3) . (4) Depletion of microglia (using clodronate) during developmental retinal vascularization interferes with this process (Fig. 5) . Endothelial cell demise is unlikely to affect microglia viability because clodronate does not elicit EC death (Fig. 4) ; this inference is consistent with other reports that, on the contrary, suggest a role for microglia in EC survival. 54 55 (5) Intravitreal injection of microglia into microglia-depleted retinas restores vascularity in the developing tissue (Fig. 6) . Collectively, these observations point to a significant involvement of microglia in retinal blood vessel formation. 
Although the precise mediator(s) underlying the functional interaction between microglia and ECs in retinal blood vessel formation is unknown, one could surmise a possible role for a number of growth factors, matrix metalloproteinases, and cytokines produced by microglia. For instance, in O2-induced retinopathy, hyperoxia has been shown to decrease proangiogenic TNF-α levels in the retina at P12, 56 which corresponds to the time of microglial and microvascular loss (Fig. 2A) . Conversely, the ensuing hypoxia that follows the hyperoxic period in this model is associated with an elevation of microglial TNF-α and coincides with the intravitreal neovascular phase. 56 57 In addition, because microglia have been shown to migrate and proliferate in response to VEGF, 58 hyperoxia may prevent these effects by decreasing VEGF levels. 
The effects of leukocytes on ocular blood vessel remodeling are complex. In contrast to findings presented herein, macrophages participate in the regression of transient vascular networks (such as the hyaloid), which are required only until permanent vessel beds are established. 59 Other leukocytes, specifically cytotoxic T lymphocytes, are also involved in retinal blood vessel remodeling and vaso-obliteration. 60 However, this does not imply that all leukocytes hinder all ocular vascular development; rather, divergent roles of macrophages in different settings have been proposed. 61 62 Hence, one can surmise that microglia are implicated in vascularization of the retina, whereas other macrophages and T lymphocytes act by pruning it. 
In summary, our data suggest that local retinal microglia have an important role in organized blood vessel formation. Therefore, if microglia elicit angiogenesis when exposed to low O2 tensions, as has been documented for macrophages, 18 it is tempting to propose their involvement in retinal blood vessel formation in response to local tissue hypoxia under physiologic 1 or pathologic 63 conditions. 
 
Figure 1.
 
Retinal wholemount histochemistry illustrating the relationship between microglia and vasculature in the developing retina. (AD) Human fetal retina (15 weeks of gestation) with lectin-stained vessels (red) and CD18-labeled microglia (green) (n = 3; representative images shown). Magnifications: (AC) ×4; (D) ×20. (EI) Six-day-old mice expressing green fluorescent protein driven by the fractalkine (CX3CL1) receptor CX3CR1 of retinal microglia (n = 4; representative images presented). Vessels were stained with lectin (red). Magnifications: (EG) ×10; (HI) ×40.
Figure 1.
 
Retinal wholemount histochemistry illustrating the relationship between microglia and vasculature in the developing retina. (AD) Human fetal retina (15 weeks of gestation) with lectin-stained vessels (red) and CD18-labeled microglia (green) (n = 3; representative images shown). Magnifications: (AC) ×4; (D) ×20. (EI) Six-day-old mice expressing green fluorescent protein driven by the fractalkine (CX3CL1) receptor CX3CR1 of retinal microglia (n = 4; representative images presented). Vessels were stained with lectin (red). Magnifications: (EG) ×10; (HI) ×40.
Figure 2.
 
Microglial expression in models of retinopathy of prematurity. (A) C57BL/6 mice were exposed to hyperoxia (75% O2) from P7 to P12. Representative retinal wholemounts (stained with lectin, which detects vessels and microglia 11 ) before and after O2 exposure are shown. Magnification: ×20. Histogram data represent means ± SEM of 20 O2-exposed retinas and 10 control retinas. *P < 0.0001 compared with control; Student t test. (B) Rats were exposed to hypercapnia (10% CO2) from within 24 hours of birth for 12 hours. Representative retinal wholemounts (labeled with lectin) before and after CO2 exposure are depicted. Magnification: ×20. Histogram data represent mean ± SEM of 6 to 9 retinas. *P = 0.0002 compared with control; Student t test.
Figure 2.
 
Microglial expression in models of retinopathy of prematurity. (A) C57BL/6 mice were exposed to hyperoxia (75% O2) from P7 to P12. Representative retinal wholemounts (stained with lectin, which detects vessels and microglia 11 ) before and after O2 exposure are shown. Magnification: ×20. Histogram data represent means ± SEM of 20 O2-exposed retinas and 10 control retinas. *P < 0.0001 compared with control; Student t test. (B) Rats were exposed to hypercapnia (10% CO2) from within 24 hours of birth for 12 hours. Representative retinal wholemounts (labeled with lectin) before and after CO2 exposure are depicted. Magnification: ×20. Histogram data represent mean ± SEM of 6 to 9 retinas. *P = 0.0002 compared with control; Student t test.
Figure 3.
 
Microglial expression in ex vivo retinal explants. Retinas of P6 rats were isolated and cultured for 3 days under control, hyperoxic (75% O2), or hypercapnic (10% CO2) conditions, with or without the superoxide dismutase mimetic CuDIPS (10 μM) or the peroxynitrite decomposition catalyst FeTPPS (10 μM). After 3 days, retinas were stained with the general microglial marker OX-42, and positive cells were counted. Representative explants are presented. Magnification: ×40. Histogram data represent mean ± SEM of 9 to 15 retinas. *P < 0.001 compared with appropriate control by one-way ANOVA followed by Bonferroni multiple comparison test.
Figure 3.
 
Microglial expression in ex vivo retinal explants. Retinas of P6 rats were isolated and cultured for 3 days under control, hyperoxic (75% O2), or hypercapnic (10% CO2) conditions, with or without the superoxide dismutase mimetic CuDIPS (10 μM) or the peroxynitrite decomposition catalyst FeTPPS (10 μM). After 3 days, retinas were stained with the general microglial marker OX-42, and positive cells were counted. Representative explants are presented. Magnification: ×40. Histogram data represent mean ± SEM of 9 to 15 retinas. *P < 0.001 compared with appropriate control by one-way ANOVA followed by Bonferroni multiple comparison test.
Figure 4.
 
Clodronate liposome effectiveness and specificity in vivo and in vitro. (A, C) Within 24 hours of birth, rats were injected intravitreally and intraperitoneally with either vehicle or clodronate liposomes. (A) Histogram data of microglial counts from retinal wholemounts labeled with lectin 5, 6, and 8 days after injection. Data are mean ± SEM of 12 retinas per group expressed as percentages relative to untreated controls. *P < 0.01 compared with vehicle alone by one-way ANOVA followed by Tukey multiple comparison test. (B) Endothelial and microglial cells were exposed to clodronate liposomes (5 μL) for 24 hours before cell viability was assessed by MTT assay. Values represent mean ± SEM of three independent experiments expressed as percentages relative to controls. *P < 0.0000025 compared with microglia group by Student t test. (C) Panels depict representative retinal sections 8 days after injection (n = 3). Magnification: ×40.
Figure 4.
 
Clodronate liposome effectiveness and specificity in vivo and in vitro. (A, C) Within 24 hours of birth, rats were injected intravitreally and intraperitoneally with either vehicle or clodronate liposomes. (A) Histogram data of microglial counts from retinal wholemounts labeled with lectin 5, 6, and 8 days after injection. Data are mean ± SEM of 12 retinas per group expressed as percentages relative to untreated controls. *P < 0.01 compared with vehicle alone by one-way ANOVA followed by Tukey multiple comparison test. (B) Endothelial and microglial cells were exposed to clodronate liposomes (5 μL) for 24 hours before cell viability was assessed by MTT assay. Values represent mean ± SEM of three independent experiments expressed as percentages relative to controls. *P < 0.0000025 compared with microglia group by Student t test. (C) Panels depict representative retinal sections 8 days after injection (n = 3). Magnification: ×40.
Figure 5.
 
Effects of microglial and macrophage depletion on developmental retinal blood vessel formation. Intravitreal and intraperitoneal clodronate liposome injections were administered to rats within 24 hours of birth. Animals were euthanatized 5, 6, and 8 days after treatment, retinas were isolated and wholemounted to assess vascularity, and blood vessels were stained with lectin, which also detects microglia. 11 (A) Representative retinal wholemounts 6 days after treatment; retinal and ciliary microvessels are clearly detectable. Dotted lines delimit the retinal vascular front. Magnification: ×4. (B) Representative portions of retinal wholemounts depicting vessel density 6 days after treatment. Magnification: ×20. (A, B) Histogram data represent mean ± SEM of 8 to 17 retinas. *P < 0.001 compared with control; one-way ANOVA followed by Bonferroni multiple comparison test.
Figure 5.
 
Effects of microglial and macrophage depletion on developmental retinal blood vessel formation. Intravitreal and intraperitoneal clodronate liposome injections were administered to rats within 24 hours of birth. Animals were euthanatized 5, 6, and 8 days after treatment, retinas were isolated and wholemounted to assess vascularity, and blood vessels were stained with lectin, which also detects microglia. 11 (A) Representative retinal wholemounts 6 days after treatment; retinal and ciliary microvessels are clearly detectable. Dotted lines delimit the retinal vascular front. Magnification: ×4. (B) Representative portions of retinal wholemounts depicting vessel density 6 days after treatment. Magnification: ×20. (A, B) Histogram data represent mean ± SEM of 8 to 17 retinas. *P < 0.001 compared with control; one-way ANOVA followed by Bonferroni multiple comparison test.
Figure 6.
 
Effects of retinal microglial compared with systemic macrophage depletion on vascularity. Intravitreal (iv) and intraperitoneal (ip) clodronate liposomes were administered to rats within 24 hours of birth, and retinas were isolated, stained with lectin, and wholemounted to assess vascularity. (A) Histogram data represent mean ± SEM of 8 to 14 retinas 6 days after injection. P < 0.01 compared with all other groups by one-way ANOVA followed by Tukey multiple comparison test (with the exception of the nonsignificant [NS] comparisons noted). (B) Microglia was injected in rats 6 days after clodronate liposome treatment; microglia or vehicle was injected intravitreally. Animals were euthanatized at P10 (4 days after receiving microglia). Representative portions of retinal wholemounts are depicted. Magnification: ×10. Histogram data represent mean ± SEM of 8 to 18 retinas. *P < 0.0013 compared with appropriate control by Student t test.
Figure 6.
 
Effects of retinal microglial compared with systemic macrophage depletion on vascularity. Intravitreal (iv) and intraperitoneal (ip) clodronate liposomes were administered to rats within 24 hours of birth, and retinas were isolated, stained with lectin, and wholemounted to assess vascularity. (A) Histogram data represent mean ± SEM of 8 to 14 retinas 6 days after injection. P < 0.01 compared with all other groups by one-way ANOVA followed by Tukey multiple comparison test (with the exception of the nonsignificant [NS] comparisons noted). (B) Microglia was injected in rats 6 days after clodronate liposome treatment; microglia or vehicle was injected intravitreally. Animals were euthanatized at P10 (4 days after receiving microglia). Representative portions of retinal wholemounts are depicted. Magnification: ×10. Histogram data represent mean ± SEM of 8 to 18 retinas. *P < 0.0013 compared with appropriate control by Student t test.
The authors thank Karl Hansford for his skillful assistance in preparing the clodronate liposomes and Hendrika Fernandez for her valuable technical support. 
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Figure 1.
 
Retinal wholemount histochemistry illustrating the relationship between microglia and vasculature in the developing retina. (AD) Human fetal retina (15 weeks of gestation) with lectin-stained vessels (red) and CD18-labeled microglia (green) (n = 3; representative images shown). Magnifications: (AC) ×4; (D) ×20. (EI) Six-day-old mice expressing green fluorescent protein driven by the fractalkine (CX3CL1) receptor CX3CR1 of retinal microglia (n = 4; representative images presented). Vessels were stained with lectin (red). Magnifications: (EG) ×10; (HI) ×40.
Figure 1.
 
Retinal wholemount histochemistry illustrating the relationship between microglia and vasculature in the developing retina. (AD) Human fetal retina (15 weeks of gestation) with lectin-stained vessels (red) and CD18-labeled microglia (green) (n = 3; representative images shown). Magnifications: (AC) ×4; (D) ×20. (EI) Six-day-old mice expressing green fluorescent protein driven by the fractalkine (CX3CL1) receptor CX3CR1 of retinal microglia (n = 4; representative images presented). Vessels were stained with lectin (red). Magnifications: (EG) ×10; (HI) ×40.
Figure 2.
 
Microglial expression in models of retinopathy of prematurity. (A) C57BL/6 mice were exposed to hyperoxia (75% O2) from P7 to P12. Representative retinal wholemounts (stained with lectin, which detects vessels and microglia 11 ) before and after O2 exposure are shown. Magnification: ×20. Histogram data represent means ± SEM of 20 O2-exposed retinas and 10 control retinas. *P < 0.0001 compared with control; Student t test. (B) Rats were exposed to hypercapnia (10% CO2) from within 24 hours of birth for 12 hours. Representative retinal wholemounts (labeled with lectin) before and after CO2 exposure are depicted. Magnification: ×20. Histogram data represent mean ± SEM of 6 to 9 retinas. *P = 0.0002 compared with control; Student t test.
Figure 2.
 
Microglial expression in models of retinopathy of prematurity. (A) C57BL/6 mice were exposed to hyperoxia (75% O2) from P7 to P12. Representative retinal wholemounts (stained with lectin, which detects vessels and microglia 11 ) before and after O2 exposure are shown. Magnification: ×20. Histogram data represent means ± SEM of 20 O2-exposed retinas and 10 control retinas. *P < 0.0001 compared with control; Student t test. (B) Rats were exposed to hypercapnia (10% CO2) from within 24 hours of birth for 12 hours. Representative retinal wholemounts (labeled with lectin) before and after CO2 exposure are depicted. Magnification: ×20. Histogram data represent mean ± SEM of 6 to 9 retinas. *P = 0.0002 compared with control; Student t test.
Figure 3.
 
Microglial expression in ex vivo retinal explants. Retinas of P6 rats were isolated and cultured for 3 days under control, hyperoxic (75% O2), or hypercapnic (10% CO2) conditions, with or without the superoxide dismutase mimetic CuDIPS (10 μM) or the peroxynitrite decomposition catalyst FeTPPS (10 μM). After 3 days, retinas were stained with the general microglial marker OX-42, and positive cells were counted. Representative explants are presented. Magnification: ×40. Histogram data represent mean ± SEM of 9 to 15 retinas. *P < 0.001 compared with appropriate control by one-way ANOVA followed by Bonferroni multiple comparison test.
Figure 3.
 
Microglial expression in ex vivo retinal explants. Retinas of P6 rats were isolated and cultured for 3 days under control, hyperoxic (75% O2), or hypercapnic (10% CO2) conditions, with or without the superoxide dismutase mimetic CuDIPS (10 μM) or the peroxynitrite decomposition catalyst FeTPPS (10 μM). After 3 days, retinas were stained with the general microglial marker OX-42, and positive cells were counted. Representative explants are presented. Magnification: ×40. Histogram data represent mean ± SEM of 9 to 15 retinas. *P < 0.001 compared with appropriate control by one-way ANOVA followed by Bonferroni multiple comparison test.
Figure 4.
 
Clodronate liposome effectiveness and specificity in vivo and in vitro. (A, C) Within 24 hours of birth, rats were injected intravitreally and intraperitoneally with either vehicle or clodronate liposomes. (A) Histogram data of microglial counts from retinal wholemounts labeled with lectin 5, 6, and 8 days after injection. Data are mean ± SEM of 12 retinas per group expressed as percentages relative to untreated controls. *P < 0.01 compared with vehicle alone by one-way ANOVA followed by Tukey multiple comparison test. (B) Endothelial and microglial cells were exposed to clodronate liposomes (5 μL) for 24 hours before cell viability was assessed by MTT assay. Values represent mean ± SEM of three independent experiments expressed as percentages relative to controls. *P < 0.0000025 compared with microglia group by Student t test. (C) Panels depict representative retinal sections 8 days after injection (n = 3). Magnification: ×40.
Figure 4.
 
Clodronate liposome effectiveness and specificity in vivo and in vitro. (A, C) Within 24 hours of birth, rats were injected intravitreally and intraperitoneally with either vehicle or clodronate liposomes. (A) Histogram data of microglial counts from retinal wholemounts labeled with lectin 5, 6, and 8 days after injection. Data are mean ± SEM of 12 retinas per group expressed as percentages relative to untreated controls. *P < 0.01 compared with vehicle alone by one-way ANOVA followed by Tukey multiple comparison test. (B) Endothelial and microglial cells were exposed to clodronate liposomes (5 μL) for 24 hours before cell viability was assessed by MTT assay. Values represent mean ± SEM of three independent experiments expressed as percentages relative to controls. *P < 0.0000025 compared with microglia group by Student t test. (C) Panels depict representative retinal sections 8 days after injection (n = 3). Magnification: ×40.
Figure 5.
 
Effects of microglial and macrophage depletion on developmental retinal blood vessel formation. Intravitreal and intraperitoneal clodronate liposome injections were administered to rats within 24 hours of birth. Animals were euthanatized 5, 6, and 8 days after treatment, retinas were isolated and wholemounted to assess vascularity, and blood vessels were stained with lectin, which also detects microglia. 11 (A) Representative retinal wholemounts 6 days after treatment; retinal and ciliary microvessels are clearly detectable. Dotted lines delimit the retinal vascular front. Magnification: ×4. (B) Representative portions of retinal wholemounts depicting vessel density 6 days after treatment. Magnification: ×20. (A, B) Histogram data represent mean ± SEM of 8 to 17 retinas. *P < 0.001 compared with control; one-way ANOVA followed by Bonferroni multiple comparison test.
Figure 5.
 
Effects of microglial and macrophage depletion on developmental retinal blood vessel formation. Intravitreal and intraperitoneal clodronate liposome injections were administered to rats within 24 hours of birth. Animals were euthanatized 5, 6, and 8 days after treatment, retinas were isolated and wholemounted to assess vascularity, and blood vessels were stained with lectin, which also detects microglia. 11 (A) Representative retinal wholemounts 6 days after treatment; retinal and ciliary microvessels are clearly detectable. Dotted lines delimit the retinal vascular front. Magnification: ×4. (B) Representative portions of retinal wholemounts depicting vessel density 6 days after treatment. Magnification: ×20. (A, B) Histogram data represent mean ± SEM of 8 to 17 retinas. *P < 0.001 compared with control; one-way ANOVA followed by Bonferroni multiple comparison test.
Figure 6.
 
Effects of retinal microglial compared with systemic macrophage depletion on vascularity. Intravitreal (iv) and intraperitoneal (ip) clodronate liposomes were administered to rats within 24 hours of birth, and retinas were isolated, stained with lectin, and wholemounted to assess vascularity. (A) Histogram data represent mean ± SEM of 8 to 14 retinas 6 days after injection. P < 0.01 compared with all other groups by one-way ANOVA followed by Tukey multiple comparison test (with the exception of the nonsignificant [NS] comparisons noted). (B) Microglia was injected in rats 6 days after clodronate liposome treatment; microglia or vehicle was injected intravitreally. Animals were euthanatized at P10 (4 days after receiving microglia). Representative portions of retinal wholemounts are depicted. Magnification: ×10. Histogram data represent mean ± SEM of 8 to 18 retinas. *P < 0.0013 compared with appropriate control by Student t test.
Figure 6.
 
Effects of retinal microglial compared with systemic macrophage depletion on vascularity. Intravitreal (iv) and intraperitoneal (ip) clodronate liposomes were administered to rats within 24 hours of birth, and retinas were isolated, stained with lectin, and wholemounted to assess vascularity. (A) Histogram data represent mean ± SEM of 8 to 14 retinas 6 days after injection. P < 0.01 compared with all other groups by one-way ANOVA followed by Tukey multiple comparison test (with the exception of the nonsignificant [NS] comparisons noted). (B) Microglia was injected in rats 6 days after clodronate liposome treatment; microglia or vehicle was injected intravitreally. Animals were euthanatized at P10 (4 days after receiving microglia). Representative portions of retinal wholemounts are depicted. Magnification: ×10. Histogram data represent mean ± SEM of 8 to 18 retinas. *P < 0.0013 compared with appropriate control by Student t test.
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