Select QPCR time-course results are shown in
Figure 3 . Among those genes that were increased by array in the glaucoma model, but not significantly after transection, we confirmed this specificity for glaucoma in five genes. First, endothelin receptor B (
Ednrb) was elevated in glaucoma at 4 of 6 time points in QPCR data (
P < 0.05 at 1D, 3D, 7D, and 4W [
Fig. 3A ]; for this gene and others noted, if results are not presented, the significance level did not reach
P = 0.05 [
Appendix 7,
P values for QPCR]). Similarly,
Edn2 was increased only in glaucoma (
P < 0.05 at 1D, 1W, and 4W, and
P < 0.01 at 2W;
Fig. 3C ).
Ednra (endothelin receptor A) was not significantly changed in either model
(Fig. 3B) . Another significantly upregulated gene in the glaucoma model alone was tumor necrosis factor receptor superfamily, member 1A (
Tnfrsf1a). Its expression peaked at 1D (
P = 0.001) and decreased thereafter
(Fig. 3B) . None of the
Tnfrsf1a QPCR values for transection eyes were significant, and the glaucoma group was significantly greater than the transection group data for this gene at 3D and 4W (
P = 0.05 and 0.02). Annexin A3 (
Anxa3) was also elevated only in glaucoma, at 4 of 6 time points: 3D (
P = 0.01), 1W, 2W, and 4W (all
P < 0.05;
Fig. 3P ). The related gene,
Anxa1, followed a similar trend, but none of the QPCR values were significantly different from control in either model
(Fig. 3O) . The final gene in this group,
Lcn2, was significantly increased in glaucoma at 1D, 1W, 2W, and 4W (all
P < 0.01) but only at 3D (
P = 0.01;
Fig. 3I ) after transection.