Antibodies raised against the following proteins were used: α-smooth muscle actin (α-SMA), vinculin, tubulin, and fibronectin (Sigma, Taufkirchen, Germany), focal adhesion kinase (FAK; Cell Signaling Technology/NEB, Frankfurt, Germany), pY397FAK (Biosource, Nivelles, Belgium), extracellular regulated kinase (ERK; Santa Cruz Biotechnology, Santa Cruz, CA), pERK (Promega, Mannheim, Germany), GAPDH, myocilin (affinity-purified goat anti-human; R&D Systems, Wiesbaden, Germany), α-B-crystallin (Stressgen/Biomol, Hamburg, Germany), fluorescent dye (Alexa 488)-conjugated goat anti-mouse (Molecular Probes, Eugene, OR), and horseradish-peroxidase (HRP)-conjugated secondary antibodies (Jackson/Dianova, Hamburg, Germany). Phalloidin-TRITC (Sigma, Taufkirchen, Germany) was used to stain filamentous actin.