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Hiroki Imai, Kouichi Ohta, Akiko Yoshida, Satoru Suzuki, Kiyoshi Hashizume, Shinichi Usami, Takanobu Kikuchi; μ-Crystallin, New Candidate Protein in Endotoxin-Induced Uveitis. Invest. Ophthalmol. Vis. Sci. 2010;51(7):3554-3559. doi: https://doi.org/10.1167/iovs.09-3728.
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© ARVO (1962-2015); The Authors (2016-present)
μ-Crystallin (CRYM) is a major taxon-specific lens protein. The purpose of this study was to investigate the function of CRYM in eyes of mice with endotoxin-induced uveitis (EIU).
EIU was induced by an injection of a lipopolysaccharide (LPS) into the footpad of male C57BL/6J, CRYM knockout (CRYM−/−), and wild-type (CRYM+/+) mice. The expression of CRYM in the iris-ciliary body (ICB) was investigated by Western blot analyses and real-time RT-PCR at 12 hours and 1, 3, and 5 days after the LPS injection. The number of cells that had infiltrated the anterior chamber (AC) of the CRYM+/+ mice was compared to that in the CRYM−/− mice at 1, 3, 5, and 7 days. The expressions of the mRNA of interleukin (IL)-1α, IL-6, tumor necrosis factor (TNF)-α, and granulocyte macrophage–colony stimulating factor (GM-CSF) in the ICB of the two groups of mice were compared.
The mRNA of CRYM was upregulated at 12 hours after LPS injection, and CRYM protein increased at 3 days. The number of inflammatory cells in the AC of the CRYM−/− mice was not significantly different on day 1 from that in the CRYM+/+ mice, but was significantly lower (17.9 ± 1.6 vs. 27.1 ± 2.4 cells/section) on day 5. Expression of the mRNA of IL-1α and -6 in the CRYM−/− mice was significantly lower than that in the CRYM+/+ mice on day 5.
CRYM plays an important role in the development of the second peak of murine EIU.
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