Multistage, stratified, cluster random sampling with probability proportional to size (PPS) procedures were used to identify a cross-sectional, nationally representative sample. The sample covered all 36 states and the Federal Capital Territory of Abuja. The population included in the sampling frame in each state was based on the actual population of the state, such that more populous states had more clusters compared with less populated states. In each cluster location, the center of the cluster was located, and a random start made by spinning a bottle. The enumeration then started from the first inhabited house on the left hand-side. The enumeration process continued until 50 adults aged ≥40 years, normally resident (continuous residence for at least the past 3 months) were identified. In the last household, if more than one eligible adult was available, all were included, even if the total exceeded 50. In small villages, if there were fewer than 50 eligible adults, the nearest village was included. Separate sampling frames were constructed for urban and rural areas, and clusters were identified randomly for urban and rural areas separately. The process resulted in identification of 310 clusters across the country, of which 226 were rural and 84 were urban.
After obtaining written informed consent, we collected personal and demographic data of all eligible participants at the time of enumeration, and the participants were invited to attend a convenient location within the cluster for clinical examination. Enumerated individuals who did not report to the examination site were followed up three times and offered an examination at their homes in the final instance before they were deemed nonrespondents. Nonrespondents were not replaced.