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Nancy J. Reyes, Elizabeth Mayhew, Peter W. Chen, Jerry Y. Niederkorn; γδ T Cells Are Required for Maximal Expression of Allergic Conjunctivitis. Invest. Ophthalmol. Vis. Sci. 2011;52(5):2211-2216. doi: https://doi.org/10.1167/iovs.10-5959.
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To determine the function of γδ T cells in early- and late-phase responses in allergic conjunctivitis.
Wild-type (WT) C57BL/6 and γδ T cell–deficient (TCR-δ−/−) mice were immunized intraperitoneally and challenged topically for 7 consecutive days with short ragweed pollen. Natural killer T (NKT) and γδ T cell–double-deficient mice were generated by treating TCR-δ−/− mice with anti-CD1d antibody. Allergic conjunctivitis was evaluated clinically, and the late-phase response was assessed by histopathology. Cytokine profiles were evaluated by ELISA. The afferent and efferent arms of allergic conjunctivitis were assessed by adoptive transfer of CD4+ T cells from WT or TCR-δ−/− mice into naive TCR-δ−/− or WT mice.
TCR-δ−/− mice had decreased clinical manifestations of allergic conjunctivitis compared with WT mice. TCR-δ−/− mice had decreased eosinophilic infiltration compared with WT mice. TCR-δ−/− mice produced less Th2-associated cytokines interleukin (IL)-4, -5, and -13 compared with WT mice. Clinical manifestations of allergic conjunctivitis were lowest in NKT cell–depleted TCR-δ−/− mice. However, late-phase allergic conjunctivitis in NKT cell–depleted, TCR-δ−/− mice was the same as TCR-δ−/− mice. Adoptive transfer of CD4+ T cells revealed that γδ T cells are needed for the afferent and efferent arms of allergic conjunctivitis.
γδ T cells are needed for full expression of both the clinical manifestations and the late phase of allergic conjunctivitis. Thus, γδ T cells have an important impact in the expression of allergic conjunctivitis and are a potential therapeutic target in the management of allergic diseases of the ocular surface.
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