Human cadaver eyes, free from glaucoma or other eye diseases, were obtained from the National Disease Research Interchange (NDRI, Philadelphia, PA) and were received in moist chambers within 48 hours of death. Ages of the seven female and seven male donors ranged from 58 to 93 years (75 ± 10.5 years [mean ± SD]). Whole eyes were fixed in 4% paraformaldehyde, embedded in paraffin, and cut into 5-μm–thick sections. Sections were placed on glass slides, deparaffinized, and rehydrated through graded alcohols. After treatment with 3% hydrogen peroxide and blocking with 5% normal rabbit serum (Jackson ImmunoResearch, West Grove, PA), sections were incubated overnight at 4°C with either goat anti–human IL-8 antibody (R&D Systems, Minneapolis, MN) or purified goat IgG (R&D Systems). Immunodetection was performed with biotinylated rabbit anti–goat secondary antibody and peroxidase-labeled streptavidin (Jackson ImmunoResearch), with red/brown chromogen (Nova Red; Vector Laboratories, Burlingame, CA). The slides were then counterstained in hematoxylin. Phase-contrast microscopy was performed with a microscope (model AX70; Olympus, Center Valley, PA) and a digital camera controlled with accompanying software (SPOT; Diagnostic Instruments, Sterling Heights, MD).