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Hong Nian, Hui Shao, Guoming Zhang, Willi K. Born, Rebecca L. O'Brien, Henry J. Kaplan, Deming Sun; Regulatory Effect of γδ T Cells on IL-17+ Uveitogenic T Cells. Invest. Ophthalmol. Vis. Sci. 2010;51(9):4661-4667. doi: https://doi.org/10.1167/iovs.09-5045.
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To characterize the regulatory effect of γδ T cells in the activation of IL-17+ uveitogenic T cells.
The authors administered the γδ TCR-specific antibody GL3 to B6 mice before or after antigen immunization and examined Th1- or Th17-polarized T-cell responses. The intensity of Th17 responses was also examined in responder T cells containing varying numbers of γδ T cells.
GL3 treatment resulted in varying degrees of depletion of circulating γδ T cells, depending on when the antibody was administered. The intensity of the αβTCR+IL-17+, but not the αβTCR+IFN-γ+, IRBP-specific T-cell responses was correlated to the percentage of γδ T cells in the responder T cells. Kinetic studies showed that early IL-17+ T cells were primarily γδ T cells, with a later gradual shift to αβ T cells. A close association was seen between the intensity of the IL-17+ autoreactive T-cell response and the percentage of γδ T cells in the responder T cells. Although a modest increase in γδ T cells among the responder T cells promoted the expansion of IL-17+ αβTCR+ T cells, a higher proportion of γδ T cells inhibited it.
γδ T cells are actively involved in the generation of αβTCR+IL-17+ T cells. The number of γδ T cells and the αβ/γδ T-cell ratio in the responder T cells regulate the intensity of the Th17-type autoreactive T-cell response.
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