Comparative analysis was performed of the intensity of the contrast agents compared with the surrounding tissues and baseline physiologic reflectivity. All the contrast agents, when used topically, had significantly increased mean intensity compared with standard non-lipid–based artificial tears (
P < 0.01;
Fig. 5A). Mean intensity values for artificial tears, PA, LBAT, and TA were 54 U, 143 U, 150 U, and 117 U, respectively. Additionally, maximum intensity was significantly increased compared with control (
P < 0.05). When compared with each other, topical PA and LBAT both had higher mean intensity than TA (
P < 0.01). There were no differences between the maximum intensity of the topical agents. Intracameral contrast agents had significantly increased mean and maximum intensity compared with aqueous and corneal stroma (
P < 0.001;
Fig. 5B). Mean intensity values for aqueous, corneal stroma, PA, LBAT, and TA were 42 U, 52 U, 106 U, 89 U, and 106 U, respectively. Intracameral comparison of the contrast agents revealed that PA had a higher mean intensity than LBAT and TA (
P < 0.05). Both TA and PA had a higher maximum intensity than LBAT (
P < 0.05). Each of the test agents also significantly increased the mean intensity of the wound interface (
P < 0.02;
Fig. 5C) and increased maximum intensity of the wound interface (
P < 0.001). Mean intensity of the PA control and PA were 70 U and 103 U, respectively. Mean intensity of LBAT-control and LBAT were 74 U and 101 U, respectively. Mean intensity of the TA control and TA were 82 U and 107 U, respectively. No significant differences were found between the agents when used within the wound interface.