Mice were anesthetized with 64 mg/kg of sodium pentobarbital. Mydriasis was induced by administration of 1 μL of 0.5% Mydrin-P (tropicamide-phenylephrine combination) drops (Santen Pharmaceutical Co., Ltd., Osaka, Japan). The drops were gently massaged into the eye by artificially blinking the eyelids. Eyes were then immediately covered with Systane Ultra artificial tears (Alcon Laboratories, Inc., Ft. Worth, TX). Mice were then placed in a warmed, humidified, oxygenated acrylic plastic sheet chamber for a minimum of 5 minutes to permit time for pupil dilation. Mice were then removed for imaging by scanning laser ophthalmoscopy (SLO) (model HRA2; Heidelberg Engineering, Inc., Vista, CA) and spectral-domain optical coherence tomography (SDOCT) (model Envisu SDOIS; Bioptigen, Inc., Research Triangle Park, NC). The SLO imaging involved collection of different imaging modalities, including dark-field reflectance and autofluorescent images with both blue (488 nm) and infrared (795 nm and 830 nm) illumination wavelengths. Using a wide-field objective lens with a 55° field of view (FOV), retinal images were collected with the optic nerve centrally positioned. Additional views of the peripheral regions were obtained to further investigate the nasal, temporal, superior, and inferior quadrants. Eyes were occasionally rehydrated with balanced salt solution or Systane Ultra artificial ears (Alcon Laboratories) and mechanically massaged to simulate blinking as needed. After SLO imaging, the mouse was transferred to the Envisu SDOIS system (Bioptigen, Inc.) for SDOCT imaging. The SDOCT volumetric scans (250 a-scans per b-scan × 250 b-scans per volume) were obtained with the optic nerve centrally located within the FOV. Using a 50° objective lens, the SDOIS system afforded a retinal FOV of approximately 1.5 mm, with an axial, in-depth resolution of approximately 6 μm. After imaging, both eyes received bacitracin zinc and polymyxin B sulfate ophthalmic ointment (Bausch & Lomb, Inc., Tampa, FL) to prevent corneal dehydration. During recovery, mice were placed in a bottom-warmed (33–36°C), oxygenated (21%–60%) acrylic plastic sheet chamber until they fully recovered from general anesthesia.