Samples were analyzed using a chemiluminescence-based ELISA for high sensitivity detection. Q-Plex human cytokine-screen (Quansys Biosciences, West Logan, UT) was used to measure the concentrations of IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-13, IL-15, IL-17, IL-23, TNF-α, lymphotoxin-α, and IFN-γ in the aqueous samples. The levels of monocyte chemotactic protein 1 (MCP-1), VEGF, and eotaxin-2 were measured with ELISA kits (PeproTech, Rocky Hill, NJ; R&D Systems, Minneapolis, MN; Antigenix America, Franklin Square, NY, respectively). Aqueous humor samples were diluted 5-fold using the diluent according to the manufacturers' instructions, and incubated on the capture antibody-coated plates overnight at 4°C and processed for ELISA detection.