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Sho C. Takatori, Percy Lazon de la Jara, Brien Holden, Klaus Ehrmann, Arthur Ho, Clayton J. Radke; In Vivo Oxygen Uptake into the Human Cornea. Invest. Ophthalmol. Vis. Sci. 2012;53(10):6331-6337. https://doi.org/10.1167/iovs.12-10059.
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© ARVO (1962-2015); The Authors (2016-present)
We provide a new procedure to quantify in situ corneal oxygen uptake using the micropolarographic Clark electrode.
Traditionally, upon placing a membrane-covered Clark microelectrode onto a human cornea, the resulting polarographic signal is interpreted as the oxygen partial pressure at the anterior corneal surface. However, the Clark electrode operates at a limiting current. Hence, oxygen flux is directly detected rather than partial pressure. We corrected this misunderstanding and devised a new analysis to quantify oxygen uptake into the cornea. The proposed analysis is applied to new polarographic data for 10 human subjects during open-eye oxygen uptake.
Average open-eye corneal oxygen uptake over 10 subjects is approximately 11 μL/(cm2 h), approximately five times larger than the average reported by researchers who invoke the original mathematical analysis. Application of the classical interpretation scheme to our experimental data also garners uptake values that are approximately a factor of three to five times smaller than those obtained with our new procedure.
The classical procedure originally developed by Fatt and colleagues misinterprets the behavior of the Clark microelectrode. We corrected the analysis of the in situ polarographic technique to provide a simple yet rigorous procedure for analyzing both previous data in the literature and those newly obtained. Our proposed interpretation scheme thus provides a reliable tool for in vivo assessment of corneal oxygen uptake.
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