Purchase this article with an account.
Xiao-Qin Wang, Hai-Lin Liu, Gui-Bo Wang, Peng-Fei Wu, Tian Yan, Jia Xie, Yong Tang, Lu-Kun Sun, Chen Li; Effect of Artesunate on Endotoxin-Induced Uveitis in Rats. Invest. Ophthalmol. Vis. Sci. 2011;52(2):916-919. doi: https://doi.org/10.1167/iovs.10-5892.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
The purpose of this study was to explore the effect of artesunate on endotoxin-induced uveitis (EIU) in rats.
EIU was induced in male Long-Evans rats by footpad injection of lipopolysaccharide (LPS 200 μg in 0.1 mL PBS; LPS-only group [LG]). PBS (0.1 mL), prednisolone (10 mg/kg in 0.1 mL PBS; prednisolone group [PG]), and artesunate of three concentrations (1, 10, 100 mg/kg; artesunate groups A, B, C [AGA, AGB, AGC]; all in 0.1 mL of PBS) were injected intravenously, respectively, 1 hour after LPS injection. Twenty-four hours after LPS injection, infiltrating cells and protein concentration in the aqueous humor, as well as tumor necrosis factor (TNF)-α, nitric oxide (NO), prostaglandin E2 (PGE2), and monocyte chemoattractant protein (MCP)-1, were measured.
Rats in AGA did not have significant difference in infiltrating cell number (61.6 × 105 cells/mL; range, 20.0–147.8 cells/mL; P = 0.287), protein concentration (48.0 mg/mL; range, 18.0–101.8 mg/mL; P = 0.349), NO concentration (86.9 μM/L; range, 30.5–192.5 μM/L; P = 0.363), but had significant difference in TNF-α (1144.3 pg/mL; range, 460.0–1840.0 pg/mL; P = 0.038), PGE2 (12.8 ng/mL; range, 4.8–27.2 ng/mL; P = 0.005), and MCP-1 (6136 pg/mL; range, 20.0–147.8 pg/mL; P = 0.009) with those of LG. Artesunate at 10 mg/kg and 100 mg/kg significantly suppressed the infiltrating cells, protein concentration, TNF-α, NO, PGE2, and MCP-1 in the aqueous humor induced by LPS (P = 0.011–0.000).
Dose-dependent decreases of infiltrating cells, protein concentration, TNF-α, PGE2, NO, and MCP-1 in the aqueous humor by artesunate treatment after LPS injection indicate that artesunate can suppress the inflammation of EIU by inhibiting the production of inflammatory mediators.
This PDF is available to Subscribers Only