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Xiao-Qin Wang, Hai-Lin Liu, Gui-Bo Wang, Peng-Fei Wu, Tian Yan, Jia Xie, Yong Tang, Lu-Kun Sun, Chen Li; Effect of Artesunate on Endotoxin-Induced Uveitis in Rats. Invest. Ophthalmol. Vis. Sci. 2011;52(2):916-919. doi: 10.1167/iovs.10-5892.
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The purpose of this study was to explore the effect of artesunate on endotoxin-induced uveitis (EIU) in rats.
EIU was induced in male Long-Evans rats by footpad injection of lipopolysaccharide (LPS 200 μg in 0.1 mL PBS; LPS-only group [LG]). PBS (0.1 mL), prednisolone (10 mg/kg in 0.1 mL PBS; prednisolone group [PG]), and artesunate of three concentrations (1, 10, 100 mg/kg; artesunate groups A, B, C [AGA, AGB, AGC]; all in 0.1 mL of PBS) were injected intravenously, respectively, 1 hour after LPS injection. Twenty-four hours after LPS injection, infiltrating cells and protein concentration in the aqueous humor, as well as tumor necrosis factor (TNF)-α, nitric oxide (NO), prostaglandin E2 (PGE2), and monocyte chemoattractant protein (MCP)-1, were measured.
Rats in AGA did not have significant difference in infiltrating cell number (61.6 × 105 cells/mL; range, 20.0–147.8 cells/mL; P = 0.287), protein concentration (48.0 mg/mL; range, 18.0–101.8 mg/mL; P = 0.349), NO concentration (86.9 μM/L; range, 30.5–192.5 μM/L; P = 0.363), but had significant difference in TNF-α (1144.3 pg/mL; range, 460.0–1840.0 pg/mL; P = 0.038), PGE2 (12.8 ng/mL; range, 4.8–27.2 ng/mL; P = 0.005), and MCP-1 (6136 pg/mL; range, 20.0–147.8 pg/mL; P = 0.009) with those of LG. Artesunate at 10 mg/kg and 100 mg/kg significantly suppressed the infiltrating cells, protein concentration, TNF-α, NO, PGE2, and MCP-1 in the aqueous humor induced by LPS (P = 0.011–0.000).
Dose-dependent decreases of infiltrating cells, protein concentration, TNF-α, PGE2, NO, and MCP-1 in the aqueous humor by artesunate treatment after LPS injection indicate that artesunate can suppress the inflammation of EIU by inhibiting the production of inflammatory mediators.
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