Corneal specimens were obtained by an ophthalmologist under aseptic conditions using standard clinical techniques. First, the specimens were smeared on slides and examined by potassium hydroxide (KOH) wet-mount and light microscopy. Then they were directly inoculated in potato dextrose broth (PDB; Becton Dickinson, Sparks, MD), incubated aerobically at 25°C and 110 rpm, examined daily, and discarded at 2 weeks if there was no growth present. Strains with yeast colonies were inoculated onto Sabouraud dextrose agar (SDA; Becton Dickinson) and filamentous colonies onto potato dextrose agar (PDA; Becton Dickinson) in C-shaped streaks, and cultured at 37°C and 25°C for 3–5 days, respectively. At the same time, we recorded the morphologic characteristics of the colonies in individual fungal cultures, such as shape, color, growth, and exudation.