December 2010
Volume 51, Issue 12
Letters to the Editor  |   December 2010
Effects of Fibroblastic and Endothelial Extracellular Matrices on Corneal Endothelial Cells
Author Affiliations & Notes
  • Jules Baum
    Department of Ophthalmology, Boston University School of Medicine, Boston, Massachusetts.
Investigative Ophthalmology & Visual Science December 2010, Vol.51, 6905-6906. doi:10.1167/iovs.10-6230
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      Jules Baum; Effects of Fibroblastic and Endothelial Extracellular Matrices on Corneal Endothelial Cells. Invest. Ophthalmol. Vis. Sci. 2010;51(12):6905-6906. doi: 10.1167/iovs.10-6230.

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      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements
In their excellent recent report, published online as a recently accepted paper on July 14, 2010, Gruschwitz et al. 1 understandably omitted a 25-year-old paper of ours. 2 In it, Hseih and I isolated extracellular matrices (ECMs) from chick embryo fibroblasts and human and rabbit corneal stromal cells. These cells induced polarization and elongation of corneal endothelial cells in culture. By indirect immunofluorescence, fibronectin was seen as arrays of long fibers in fibroblastic ECM, whereas in endothelial ECM, fibronectin was found in discrete foci of short fibers. The morphology of endothelial cells in culture was associated with the structure of the ECM that was laid down: short fibers in clusters associated with a typical polygonal shape, with long polarized fibers inducing a fibroblastic appearance. 
My coworkers and I applaud the authors and look forward to their future efforts. 
Gruschwitz R Friedrichs J Valtink M . Alignment and cell-matrix interactions of human corneal endothelial cells on nanostructured collagen type 1 matrices. Invest Ophthalmol and Vis Sci. 2010;51:6303–6310. [CrossRef]
Hsieh P Baum J . Effects of fibroblastic and endothelial extracellular matrices on corneal endothelial cells. Invest Ophthalmol Vis Sci. 1985;26:457–463. [PubMed]

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