The following antibodies were used in this study: collagen I (600-401-103-0.1; Rockland, Gilbertsville, PA, USA), collagen IV (AB756P; EMD Millipore, Billerica, MA, USA), collagen VI (SAB4500387; Sigma-Aldrich, St. Louis, MO, USA), fibronectin (ab23750; Abcam, Cambridge, MA, USA), laminin (AB2034; EMD Millipore), PAI-1 (ab28207; Abcam), connective tissue growth factor (sc-14939; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), SPARC (AF942; R&D Systems, Minneapolis, MN, USA), TGF-β2 (ab36495; Abcam). Sections were washed with xylene and subsequently hydrated with ethanol dilutions (100%, 95%, 70%). Tissue then was blocked in 5% bovine serum albumin (when probing for fibronectin) or 10% donkey serum (all other antibodies) for 1 hour at room temperature. Animal serum contained fibronectin epitopes, making it incompatible with the fibronectin antibody. Tissue then was permeabilized using 0.2% Triton X-100 and primary antibody was applied at 1:100 concentration overnight at 4°C. Slides subsequently were washed with ×1 PBS-T and 1:200 secondary antibody was applied for 1 hour at room temperature. Slides were washed once again, and the tissue was imaged using the Zeiss Axiovert 200M inverted fluorescent microscope (Carl Zeiss Meditec; Heidelberg, Germany) attached to a digital camera (AxioCam MR3; Carl Zeiss Meditec). Images were obtained using the associated software (Axiovision 4.8.2; Carl Zeiss Meditec). Slides of tissue treated with Ad.empty and Ad.TGF-β2 were imaged at the same time using the same control slide to minimize background.