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GORDON I. KAYE, SAIICHI MISHIMA, JEANNE D. COLE, NANCY WEBER KAYE; Studies on the Cornea VII. Effects of Perfusion with a Ca++-free Medium on the Corneal Endothelium. Invest. Ophthalmol. Vis. Sci. 1968;7(1):53-66.
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Paired isolated corneas were perfused in vitro with a modified KEI medium according to the method of Mishima and Kudo. One eye of each pair was perfused with the complete medium and the other with a Ca++-free medium. Progressive corneal swelling occurred in the Ca++-free medium. The swollen corneas had a greatly increased passive permeability to nontransported nonelectrolytes, suggesting a physical change in the permeability barrier. Electron microscopic examination of the swollen corneas showed that the apical functional complexes (terminal bars) underwent progressive disintegration in the Ca++-free medium and that the degree of this disintegration was correlated with the degree of swelling.
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