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Mor M. Dickman, Marc P. F. H. L. van Maris, Friso W. van Marion, Yvonne Schuchard, Petra Steijger-Vermaat, Frank J. H. M. van den Biggelaar, Tos T. J. M. Berendschot, Rudy M. M. A. Nuijts; Surface Metrology and 3-Dimensional Confocal Profiling of Femtosecond Laser and Mechanically Dissected Ultrathin Endothelial Lamellae. Invest. Ophthalmol. Vis. Sci. 2014;55(8):5183-5190. doi: 10.1167/iovs.14-14309.
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To determine the feasibility of confocal profiling in measuring surface roughness and obtaining 3-dimensional reconstructions of mechanically dissected and femtosecond (fs)-laser photodisrupted endothelial lamellae. To determine the predictability of single-pass dissection of ultrathin endothelial lamellae using a novel motor-driven linear microkeratome.
Thirty (n = 30) human corneas were harvested using a motor-driven linear microkeratome (n = 20); a hand-driven rotatory microkeratome (n = 6); and a 60-kHz fs laser (n = 4). Surface roughness was measured using an optical profiler operated in confocal microscopy mode followed by environmental scanning-electron-microscopy.
Mean surface roughness for the fs laser, motor-driven linear microkeratome, and hand-driven rotatory microkeratome measured 1.90 ± 0.48 μm, 1.06 ± 0.42 μm, and 0.93 ± 0.25 μm, respectively. Femtosecond photodisrupted lamellae were significantly rougher than mechanically dissected lamellae (P < 0.001). Mean (±SD) cutting depth with the motor-driven linear microkeratome measured: 552 ± 11 μm (550-μm head); 505 ± 19 μm (550-μm head); 459 ± 19 μm (450-μm head); and 392 ± 20 μm (400-μm head).
Confocal microscopy allows quantitative surface roughness analysis and 3-dimensional reconstruction of human corneal lamellae. Femtosecond-laser photodisruption at 60 kHz results in rougher surfaces compared with mechanical dissection. The motor-driven linear microkeratome allows single-pass dissection of ultrathin endothelial lamellae with a standard deviation ≤20 μm.
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