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Yanrong Jiang, Qiang Lu, Jing Feng; The Role of Apelin in the Retina of Diabetic Rats. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1131.
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The purpose of this study was to investigate the effect of apelin-13 on proliferative potential in diabetic retinopathy (DR), and its antagonist inhibitory effects.
Localization of apelin-13, GFAP, and VEGF were detected using immunofluorescence in the retina of diabetic rats. The mRNA and protein of apelin-13, GFAP, and VEGF in the retina of diabetic rats were measured using real-time PCR and western blot. Apelin-13 antagonist F13A was used to block apelin-13, and to study its effects in vivo.
Strong staining of apelin-13, co-localized with GFAP and VEGF, was observed in the retina of diabetic rats. Apelin-13, GFAP, and VEGF mRNA and protein levels were significantly increased in the sample’s retina. Moreover, exogenous apelin-13 promoted retinal Müller cell proliferation in vivo. Simultaneously, apelin-13 induced GFAP and VEGF expression. F13A markedly reduced the retinal gliosis caused by diabetes. Furthermore, F13A suppressed both GFAP and VEGF expression in vivo.
Our results strongly suggest that apelin-13 is associated with the development of DR, and contributes to changes in the retina of diabetic rats. Apelin-induced promotion of cell proliferation lends support to the possibility that apelin-13 may play a role in the progression of DR to a proliferative phase. This possible role deserves further investigation, which may open new perspectives in the early prevention and treatment of DR.
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