Purchase this article with an account.
Daniela Bonci, Maureen Neitz, Paulo Roney Goulart, Juliana Soares, Mario Fiorani, Olavo Galvão, Ricardo Gattass, Luiz Carlos Silveira, Dora Ventura; Allelic variation of visual pigments in capuchin monkeys, Sapajus spp. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1322.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To study the variation and frequency of M/L visual pigment genes capuchin monkeys, Sapajus spp from Brazil.
blood samples were analyzed from capuchin monkeys maintained in different Brazilian cities: Belém (N=18) and Rio de Janeiro (N=13). The M/L opsin genes of all monkeys were sequenced after DNA extraction and PCR amplification procedures. The 180, 277 and 285 amino acids were identified and the spectral absorption curve was predicted for each animal. The type and frequency of visual pigment alleles were determined.
31 capuchin monkeys were evaluated for M/L genes. All males (N=21) and some females (N=5) had only one M/L allele and were identified as protanope or deuteranope dichromats. Five females showed 2 different alleles which characterized them as trichromats. For the 18 deuteranope monkeys, the SYT (λmax 560-563) or AYT (λmax 550-556) alleles were present in 15 and 3 animals, respectively. Eight monkeys had AFA (λmax 532) (N=4) or AFT (λmax 542-547) (N=4) alleles characterizing them as protanope. The trichromat females showed two alleles combinations, AFA+AFT (N=2) and SFT (λ max 546-553) + SYT (N=3). A total of 5 different alleles were described for Sapajus spp. The frequency of SYT allele (58%) was higher than AYT (12%), AFA (15%) and AFT (15%) alleles in dichromats animals. In trichromatic females, the combination SFT+SYT was more frequent (60%) than AFA+AFT combination (40%).
The present work is the first to describe 5 different M/L opsin alleles for Sapajus spp genus. The SYT allele was the most frequent, which can be explained by the evolution of mammal opsin genes.
This PDF is available to Subscribers Only