June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Spectral Domain OCT in Experimental Glaucoma
Author Affiliations & Notes
  • Carol Rasmussen
    School of Medicine and Public Health, University of Wisconsin, Madison, WI
    OSOD (Ocular Services On Demand, LLC), Madison, WI
  • Kimberly McIntyre
    Comparative Ophthalmic Research Laboratories, Madison, WI
  • Anne Goulding
    School of Medicine and Public Health, University of Wisconsin, Madison, WI
    OSOD (Ocular Services On Demand, LLC), Madison, WI
  • Chad Busse
    Covance Laboratories, Madison, WI
  • Jennifer Szurka
    Covance Laboratories, Madison, WI
  • Amanda Harvey
    Covance Laboratories, Madison, WI
  • James Ver Hoeve
    School of Medicine and Public Health, University of Wisconsin, Madison, WI
    OSOD (Ocular Services On Demand, LLC), Madison, WI
  • Christopher Murphy
    OSOD (Ocular Services On Demand, LLC), Madison, WI
    Surgical and Radiological Sciences, University of California, Davis, CA
  • Brian Christian
    Covance Laboratories, Madison, WI
  • T Michael Nork
    School of Medicine and Public Health, University of Wisconsin, Madison, WI
    OSOD (Ocular Services On Demand, LLC), Madison, WI
  • Footnotes
    Commercial Relationships Carol Rasmussen, None; Kimberly McIntyre, None; Anne Goulding, None; Chad Busse, None; Jennifer Szurka, None; Amanda Harvey, None; James Ver Hoeve, OSOD, LLC (C), Covance, Inc (F); Christopher Murphy, Ocular Services On Demand (I), Ocular Services On Demand (C), Platypus Technologies LLC (I), Imbed LLC (I), EyeKor LLC (I), Allergan (C), Genentech (C), Sarcode (C), Covance (C); Brian Christian, None; T Michael Nork, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 1455. doi:https://doi.org/
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      Carol Rasmussen, Kimberly McIntyre, Anne Goulding, Chad Busse, Jennifer Szurka, Amanda Harvey, James Ver Hoeve, Christopher Murphy, Brian Christian, T Michael Nork; Spectral Domain OCT in Experimental Glaucoma. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1455. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To evaluate retinal nerve fiber layer (RNFL) and macular thickness values in monkeys with experimentally induced glaucoma (ExpG), using Spectralis (Heidelberg Engineering, Heidelberg, Germany, s.v. 5.4.6) and Cirrus (Carl Zeiss Meditec, Dublin, CA, s.v. 6.0.1) spectral domain optical coherence tomography (SD OCT) instruments.

Methods: Six female cynomolgus macaques with ExpG OD, induced via laser scarification of the trabecular meshwork, were used. Untreated OS served as the control. RNFL, optic nerve, macula and single line scans were collected, 2 to 4 of each, using both instruments. Thickness (um) and volume (um3) values, auto-segmented by the instruments (ASeg) and/or manually segmented (MSeg), are shown as mean±sem; significance is p≤0.05, students t test.

Results: Spectralis: ASeg values in ExpG eyes were significantly lower than in controls for RNFL (53±3.4 vs 101±3.0) and macular volume (MV) (8.8±0.22 vs 9.4±0.26). ASeg central macular thickness (CMT) in ExpG eyes was not meaningfully different from controls (263±12.7 vs 245±9.6). Comparison of ExpG and control eye values following Mseg showed the same trend. Aseg overestimated Mseg average RNFL, MV and CMT more in ExpG eyes (27, 10 and 11 % ) than in controls (2, 5 and 4%). Cirrus: ASeg values in ExpG and control eyes for RNFL (52±2.4 vs 94±2.9), MV (9.6±0.25 vs 10.5±0.28) and CMT (228±7.6 vs 223±6.1) showed differences similar to Spectralis data. Macular retinal ganglion cell-inner plexiform layer (GCIPL) thickness in ExpG eyes (53±2.6) was significantly lower than in controls (84±2.8). Average control Aseg values from Spectralis were higher than values from Cirrus for RNFL (108%) and CMT (110%) but lower for MV (90%). Enhanced depth imaging (EDI) improved visualization of the choroid and optic nerve structures: the magnitude of posterior displacement of the lamina cribrosa, as well as prelaminar and laminar tissue thickness. Spectralis image quality averaged 27 for MV and CMT scans and 37 for RNFL scans. Cirrus signal strength averaged 9.6 for optic nerve and 10.0 for mac cube scans.

Conclusions: Both instruments showed significantly lower RNFL, MV and GCIPL values in ExpG eyes than controls, and comparable CMT between eyes. EDI and GCIPL features were useful in the ExpG model. Aseg, was more variable in the ExpG eyes, did not show a consistent instrument bias for all parameters, highlighting the importance of comparing treatments with the same equipment.

Keywords: 552 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • 551 imaging/image analysis: non-clinical • 610 nerve fiber layer  
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