June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Structured illumination ophthalmoscope for high-resolution fluorescence imaging of retinal pigment epithelium
Author Affiliations & Notes
  • Stefan Dithmar
    Department of Ophthalmology, University of Heidelberg, Heidelberg, Germany
  • Gerrit Best
    Department of Ophthalmology, University of Heidelberg, Heidelberg, Germany
    Kirchhoff Institute for Physics, University of Heidelberg, Heidelberg, Germany
  • Sabrina Rossberger
    Department of Ophthalmology, University of Heidelberg, Heidelberg, Germany
    Kirchhoff Institute for Physics, University of Heidelberg, Heidelberg, Germany
  • Thomas Ach
    Department of Ophthalmology, University of Heidelberg, Heidelberg, Germany
    Department of Ophthalmology, University of Alabama, Birmingham, AL
  • Stefanie Pollithy
    Department of Ophthalmology, University of Heidelberg, Heidelberg, Germany
  • Christoph Cremer
    Kirchhoff Institute for Physics, University of Heidelberg, Heidelberg, Germany
    Institute of Molecular Biology, University of Mainz, Mainz, Germany
  • Footnotes
    Commercial Relationships Stefan Dithmar, None; Gerrit Best, None; Sabrina Rossberger, None; Thomas Ach, None; Stefanie Pollithy, None; Christoph Cremer, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 1464. doi:
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    • Get Citation

      Stefan Dithmar, Gerrit Best, Sabrina Rossberger, Thomas Ach, Stefanie Pollithy, Christoph Cremer; Structured illumination ophthalmoscope for high-resolution fluorescence imaging of retinal pigment epithelium. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1464.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Structured illumination microscopy (SIM) enables high resolution light microscopy of fluorescent molecules in a 110 nanometer range. In this study, SIM was applied for fluorescence imaging of the fundus.

Methods: We developed a device for high-resolution fluorescence imaging of the retinal pigment epithelium (RPE) in the intact eye using SIM. To study the setup's imaging capabilities, we used a model eye containing RPE-choroid specimen.

Results: Lateral resolution of < 2µm and high contrast was achieved allowing us to resolve the fluorescence distribution in single cells.

Conclusions: Applicability of structured illumination funduscopy was shown using an artificial eye. It was possible to exceed the resolution of state of the art ophthalmoscopes.

Keywords: 552 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • 701 retinal pigment epithelium • 599 microscopy: light/fluorescence/immunohistochemistry  
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