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Stefan Dithmar, Gerrit Best, Sabrina Rossberger, Thomas Ach, Stefanie Pollithy, Christoph Cremer; Structured illumination ophthalmoscope for high-resolution fluorescence imaging of retinal pigment epithelium. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1464.
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Structured illumination microscopy (SIM) enables high resolution light microscopy of fluorescent molecules in a 110 nanometer range. In this study, SIM was applied for fluorescence imaging of the fundus.
We developed a device for high-resolution fluorescence imaging of the retinal pigment epithelium (RPE) in the intact eye using SIM. To study the setup's imaging capabilities, we used a model eye containing RPE-choroid specimen.
Lateral resolution of < 2µm and high contrast was achieved allowing us to resolve the fluorescence distribution in single cells.
Applicability of structured illumination funduscopy was shown using an artificial eye. It was possible to exceed the resolution of state of the art ophthalmoscopes.
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