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Michael Pircher, Franz Felberer, Julia Kroisamer, Bernhard Baumann, Stefan Zotter, Ursula Schmidt-Erfurth, Christoph Hitzenberger; Adaptive optics enhanced SLO/OCT imaging of human photoreceptors with axial eye tracking. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1508. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the feasibility of a newly developed adaptive optics scanning laser ophthalmoscope/optical coherence tomography (AO-SLO/OCT) instrument for imaging human photoreceptors in vivo.
The instrument is based on en-face OCT which allows the simultaneous recording of SLO images with a pixel to pixel correspondance betweem both imaging modalities. The SLO/OCT instrument can be operated at frame rates between 10 and 40 fps (transverse frames, imaging area ~1°x1°) and a 3D volume is acquired within a few seconds. The SLO images are used in a post-processing step to correct for transverse eye motion and allow for a direct comparison of the images retrieved with both techniques. In order to eliminate axial eye motion artifacts the en-face OCT instrument incorporates an axial high speed eye tracker which is operated at 1 kHz. Together with the post-processing the instrument provides nearly motion artifact free 3D data of the retina. Adaptive optics is operated in closed loop mode with an imaging beam diameter of 8mm which provides ~2-3µm lateral resolution for both SLO and OCT.
The human cone mosaic was observed with both imaging modalities in the fovea. Two different layers, the inner outer segment junction and the posterior tips of photoreceptors, show the cone mosaic. In addition highly backscattering intensity spots can be found that are localized between these two layers (within the layer of the cone outer segments) in all measurements.
The new instrument provides nearly motion artifact free 3D data of the human retina in vivo with high lateral resolution and allows for a direct comparison of the two imaging modalities. The high axial resolution of the OCT provides additional information that cannot be obtained otherwise.
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