June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Adaptive optics enhanced SLO/OCT imaging of human photoreceptors with axial eye tracking
Author Affiliations & Notes
  • Michael Pircher
    Center for Med Pyhs & Biomed Eng, Medical University of Vienna, Vienna, Austria
  • Franz Felberer
    Center for Med Pyhs & Biomed Eng, Medical University of Vienna, Vienna, Austria
  • Julia Kroisamer
    Center for Med Pyhs & Biomed Eng, Medical University of Vienna, Vienna, Austria
    Department of Ophthalmology and Optometry, Medical University of Vienna, Vienna, Austria
  • Bernhard Baumann
    Center for Med Pyhs & Biomed Eng, Medical University of Vienna, Vienna, Austria
  • Stefan Zotter
    Center for Med Pyhs & Biomed Eng, Medical University of Vienna, Vienna, Austria
  • Ursula Schmidt-Erfurth
    Department of Ophthalmology and Optometry, Medical University of Vienna, Vienna, Austria
  • Christoph Hitzenberger
    Center for Med Pyhs & Biomed Eng, Medical University of Vienna, Vienna, Austria
  • Footnotes
    Commercial Relationships Michael Pircher, None; Franz Felberer, None; Julia Kroisamer, None; Bernhard Baumann, Canon Inc. (F); Stefan Zotter, Canon Inc. (F); Ursula Schmidt-Erfurth, Alcon (C), Bayer Healthcare (C), Novartis (C); Christoph Hitzenberger, Canon Inc. (F), Canon Inc. (C)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 1508. doi:https://doi.org/
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      Michael Pircher, Franz Felberer, Julia Kroisamer, Bernhard Baumann, Stefan Zotter, Ursula Schmidt-Erfurth, Christoph Hitzenberger; Adaptive optics enhanced SLO/OCT imaging of human photoreceptors with axial eye tracking. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1508. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To investigate the feasibility of a newly developed adaptive optics scanning laser ophthalmoscope/optical coherence tomography (AO-SLO/OCT) instrument for imaging human photoreceptors in vivo.

Methods: The instrument is based on en-face OCT which allows the simultaneous recording of SLO images with a pixel to pixel correspondance betweem both imaging modalities. The SLO/OCT instrument can be operated at frame rates between 10 and 40 fps (transverse frames, imaging area ~1°x1°) and a 3D volume is acquired within a few seconds. The SLO images are used in a post-processing step to correct for transverse eye motion and allow for a direct comparison of the images retrieved with both techniques. In order to eliminate axial eye motion artifacts the en-face OCT instrument incorporates an axial high speed eye tracker which is operated at 1 kHz. Together with the post-processing the instrument provides nearly motion artifact free 3D data of the retina. Adaptive optics is operated in closed loop mode with an imaging beam diameter of 8mm which provides ~2-3µm lateral resolution for both SLO and OCT.

Results: The human cone mosaic was observed with both imaging modalities in the fovea. Two different layers, the inner outer segment junction and the posterior tips of photoreceptors, show the cone mosaic. In addition highly backscattering intensity spots can be found that are localized between these two layers (within the layer of the cone outer segments) in all measurements.

Conclusions: The new instrument provides nearly motion artifact free 3D data of the human retina in vivo with high lateral resolution and allows for a direct comparison of the two imaging modalities. The high axial resolution of the OCT provides additional information that cannot be obtained otherwise.

Keywords: 552 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • 648 photoreceptors • 524 eye movements: recording techniques  
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