Purchase this article with an account.
Zhen-Yang Zhao, Yiqin Zuo, Pei Xu, Bo Yu, Massoud Motamedi, Adam Boretsky, Yan Chen, Jiyang Cai; Increased Fundus Autofluorescence and Retinal Cell Infiltration in Middle-Aged Mice Fed with High Fat, Cholesterol-Rich Diet. Invest. Ophthalmol. Vis. Sci. 2013;54(15):161. doi: https://doi.org/.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Oxidative stress and high plasma cholesterol level have been postulated as contributing to the pathogenesis of various retinal diseases such as age-related macular degeneration and diabetic retinopathy. The purpose of this study is to characterize the retinal phenotype of mice on high fat, cholesterol-rich (HF-C) diet and study the underlying mechanisms that accelerate retinal aging and inflammation.
B6.129 mice at 12 months of age were fed with either standard rodent chow or HF-C diet, with 5% and 20% (w/w) fat, respectively. The HF-C diet also contained 0.15% cholesterol. Fundus phenotype of both groups was characterized using optical coherence tomography (OCT) and scanning laser ophthalmoscope (SLO) both before and after the HF-C diet for 2 months. Immunohistochemistry and immunofluorescence staining of paraffin or cryosections were performed to further validate findings from the in vivo imaging.
At beginning of the study all animals showed healthy fundus photographs and marginal autofluorescence. Two months after diet change, mice fed with HF-C food showed dome-shaped dotted retinal lesions that were absent in mice on normal diet. SLO revealed significant increase of fundus autofluorescence in HF-C group. Most of the fluorescent spots were in the focal plane of the neuroretina, usually adjacent to retinal vessels. This was also confirmed by OCT scanning. Histology sections of HF-C mice showed increased isolectin- and CD68-positive cells, which were mainly present in the inner plexiform layer as well as the subretinal space.
Feeding high HF-C diet to 12-month old mice could accelerate the retinal aging process, which was presented as elevated fundus autofluorescence. The infiltration of CD68 positive cells indicated microglia and/or macrophages activation that can lead to a pro-inflammatory milieu in the retina and contribute to age-related retinal pathology.
This PDF is available to Subscribers Only