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Guiqiu Zhao, Jing Lin, Qian Wang, Qiang Xu; The Expression and meanning of Skp2, p27, PTEN proteins in non-Hodgkin B-cell lymphoma of ocular adnexa. Invest. Ophthalmol. Vis. Sci. 2013;54(15):173.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the expression and the correlation of protein of S-phase kinase associationprotien 2(Skp2), p27 and phosphatases phosphatase and tensin homologue (PTEN) in non-Hodgkin B-cell lymphoma(NHL)of ocular adnexa.
Paraffin sections were collected from patients suffering from B cell NHL (n=30) in Qingdao Affiliated Hospital from 1995 to 2011 and lymphadenosis (n=10) of ocular adnexa as the control group. The expression of Skp2, p27 and PTEN proteins were detected by Immunohistochemistry and the positive expression rate between lymphomas and lymphadenosis was compared by χ2 inspection. Spearman rank correlation was used to estimate the relationships among three proteins in ocular lymphoma. Pathologic grade were evaluated as independent factor of clinical information.
The expression of three proteins were related to pathologic type of tumors, but not related to age, gender, pathogenetic locations. In non-Hodgkin B-cell lymphoma of ocular adnexa, the expression of Skp2(76.7%, 23/30) was higher than that in control group(0/10)( χ2=19.79, P=0.001), while the expression of p27(73.3%, 22/30) and PTEN(56.7%, 17/30) was lower than that in the control group(10/10 and 10/10) (χ2=8.37, P=0.039; χ2 =13.48,P=0.004). With the increase of pathologic grade, Skp2 labeling frequency increase gradually, while p27 and PTEN labeling frequency decrease gradually; There was a negative correlation between p27 and Skp2 in MALT(r=-0.134, p<0.05) while there was a positive correlation between p27 and PTEN(r=0.828, p<0.05). The relationship between Skp2 and PTEN was also negative (r=-0.883, p<0.05).
The high expression of Skp2 and lower expression of p27 and PTEN may play a role in the tumorigenesis and different pathologic type of the B cell NHL of ocular adnexa. The expressions of three proteins correlate with each other in mucosal-associated lymphoid tissue.
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