June 2013
Volume 54, Issue 15
Jump To...
Free
ARVO Annual Meeting Abstract  |   June 2013
Subretinal Implantation of Gelatin Films with Stem Cells Derived RPE in Rats
Yuntao Hu; Kenrick Kuwahara; Padmaja Thomas; Bruno Diniz; Ramiro Ribeiro; Ashish Ahuja; Sherry Hikita; Lincoln Johnson; Biju Thomas; Mark Humayun
Author Affiliations & Notes
  • Yuntao Hu
    Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA
    Ophthalmolgy, Peking University Third Hospital, Beijing, China
  • Kenrick Kuwahara
    Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA
  • Padmaja Thomas
    Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA
  • Bruno Diniz
    Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA
    Retina Sector, Universidade Federal de Sao Paulo, Sao Paulo, Belize
  • Ramiro Ribeiro
    Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA
    Hospital Evangelico de Curitiba, FEMPAR, Curitiba, Belize
  • Ashish Ahuja
    Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA
  • Sherry Hikita
    Center for Stem Cell Biology and Engineering, University of California-Santa Barbara, Santa Barbara, CA
  • Lincoln Johnson
    Center for Stem Cell Biology and Engineering, University of California-Santa Barbara, Santa Barbara, CA
  • Biju Thomas
    Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA
  • Mark Humayun
    Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA
  • Footnotes
    Commercial Relationships Yuntao Hu, None; Kenrick Kuwahara, None; Padmaja Thomas, None; Bruno Diniz, None; Ramiro Ribeiro, None; Ashish Ahuja, None; Sherry Hikita, None; Lincoln Johnson, None; Biju Thomas, None; Mark Humayun, Bausch & Lomb (F), Bausch & Lomb (C), Bausch & Lomb (P), Bausch & Lomb (R), Bausch & Lomb (S), Alcon (C), Alcon (R), Iridex (P), Iridex (R), Replenish (I), Replenish (C), Replenish (R), Replenish (S), Second Sight (F), Second Sight (I), Second Sight (C), Second Sight (P), Second Sight (R), Second Sight (S), Regenerative Patch Technologies (I), Regenerative Patch Technologies (C)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 1763. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Subretinal Implantation of Gelatin Films with Stem Cells Derived RPE in Rats
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      Yuntao Hu, Kenrick Kuwahara, Padmaja Thomas, Bruno Diniz, Ramiro Ribeiro, Ashish Ahuja, Sherry Hikita, Lincoln Johnson, Biju Thomas, Mark Humayun; Subretinal Implantation of Gelatin Films with Stem Cells Derived RPE in Rats. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1763.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract
 
Purpose
 

Human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cells were cultured on gelatin films and implanted into the subetinal space in rats in order to monitor post-implantation cell fate and gelatin degradation rate.

 
Methods
 

Monolayer cultures of hESC-RPE cells were cultured on 27 μm thick gelatin films. RCS rats were randomly divided into two groups and subretinally implanted with gelatin films without the cells (Group A, n=9) or gelatin films with confluent hESC-RPE cells (Group B, n=9). One week, one month and three months post-implantation OCT scanning and histological examinations were performed.

 
Results
 

The gelatin without the cells did not degrade in subretinal space up to 3 months after implantation (Fig.1). Degradation of the gelatin with hESC-RPE cells was observed at 3 months after implantation (Fig.2). However, the hESC-RPE cells maintained monolayer RPE morphology and were RPE65 positive.

 
Conclusions
 

Degradation of subretinally implanted gelatin scaffolds is cell-dependent and may be related to the release of proteolytic enzymes or other factors by hESC-RPE cells.

 
Fig.1 Gelatin film (without cells) did not degrade after subretinal implantation 3 months.
View OriginalDownload Slide
 
Fig.1 Gelatin film (without cells) did not degrade after subretinal implantation 3 months.
 
Fig.1 Gelatin film (without cells) did not degrade after subretinal implantation 3 months.
Fig.1 Gelatin film (without cells) did not degrade after subretinal implantation 3 months.
View OriginalDownload Slide
 
Fig.2 The hESC-RPE cells maintained monolayer on gelatin film after subretinal implantation 3 months. Gelatin degraded partly near to the hESC-RPE cells.
View OriginalDownload Slide
 
Fig.2 The hESC-RPE cells maintained monolayer on gelatin film after subretinal implantation 3 months. Gelatin degraded partly near to the hESC-RPE cells.
 
Fig.2 The hESC-RPE cells maintained monolayer on gelatin film after subretinal implantation 3 months. Gelatin degraded partly near to the hESC-RPE cells.
Fig.2 The hESC-RPE cells maintained monolayer on gelatin film after subretinal implantation 3 months. Gelatin degraded partly near to the hESC-RPE cells.
View OriginalDownload Slide
 
Keywords: 701 retinal pigment epithelium • 721 stem cells • 741 transplantation  
© 2013, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept