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Yuntao Hu, Kenrick Kuwahara, Padmaja Thomas, Bruno Diniz, Ramiro Ribeiro, Ashish Ahuja, Sherry Hikita, Lincoln Johnson, Biju Thomas, Mark Humayun; Subretinal Implantation of Gelatin Films with Stem Cells Derived RPE in Rats. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1763. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
Human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cells were cultured on gelatin films and implanted into the subetinal space in rats in order to monitor post-implantation cell fate and gelatin degradation rate.
Monolayer cultures of hESC-RPE cells were cultured on 27 μm thick gelatin films. RCS rats were randomly divided into two groups and subretinally implanted with gelatin films without the cells (Group A, n=9) or gelatin films with confluent hESC-RPE cells (Group B, n=9). One week, one month and three months post-implantation OCT scanning and histological examinations were performed.
The gelatin without the cells did not degrade in subretinal space up to 3 months after implantation (Fig.1). Degradation of the gelatin with hESC-RPE cells was observed at 3 months after implantation (Fig.2). However, the hESC-RPE cells maintained monolayer RPE morphology and were RPE65 positive.
Degradation of subretinally implanted gelatin scaffolds is cell-dependent and may be related to the release of proteolytic enzymes or other factors by hESC-RPE cells.
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