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Melody Dutot, Roxane Fagon, Patrice Rat; PHLOROTANNIN-RICH NATURAL EXTRACT FROM BROWN SEAWEED ASCOPHYLLUM NODOSUM PREVENTS IN VITRO HIGH GLUCOSE RETINAL DAMAGES. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1955.
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Activators of the class III histone deacetylase SIRT1, which are also potent antioxidant molecules, are considered as therapeutics for the treatment of type 2 diabetes. In this study, we investigated the protective cellular effects of phlorotannin-rich natural extract from brown seaweed ascophyllum nodosum against high glucose on retinal pigmented epithelial (RPE) cells and Müller glial cells.
RPE cells (ARPE-19 human cell line) and Müller cells (MIO-M1 human cell line) were incubated with normal (5mM) glucose or high (25mM) glucose for 48 hours. Reactive oxygen species (ROS) production, mitochondrial transmembrane potential, and SIRT1 enzymatic activity were evaluated. Phlorotannin-rich natural extract from brown seaweed ascophyllum nodosum was tested for its ability to inhibit oxidative damages and SIRT1 activity alterations induced by high glucose.
Phlorotannin-rich natural extract from brown seaweed ascophyllum nodosum prevented glucose-induced ROS overproduction and alterations of mitochondrial transmembrane potential. We observed a significant increase in SIRT1 activity with phlorotannin-rich natural extract from brown seaweed ascophyllum nodosum.
Phlorotannin-rich natural extract from brown seaweed ascophyllum nodosum protected retinal cells against oxidative stress, mitochondrial damages and SIRT1 activity alteration induced by high glucose. Ascophyllum nodosum represents a promising ingredient for functional food supplements for diabetic patients.
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