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Jeewon Mok, Choun-Ki Joo; Tear expression profiling of cytokine, chemokine and soluble receptor in keratoconus patients. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2058.
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To determine whether the expression levels of cytokines, chemokines and soluble receptor in tear of keratoconus patients contribute to the pathogenesis of keratoconus
The patients with keratoconus were diagnosed based on the following criteria: (1) symptoms of keratoconus (Munson sign, protrusion, Vogt’s striae, corneal thickness, scarring, Fleishcher ring, photokeratoscopy signs, video keratography signs, and refractive errors) and (2) medical histories (age, gender, contact lense wearing, eye rubbing, systemic disease, atopy, and connective tissue disease). Tears were collected from 28 keratoconus patients (56 eyes) and 30 healthy subjects (60 eyes) by a polyurethane minisponges. Control subjects with no history of ocular disease were also enrolled. The concentrations of cytokines/chemokines were analyzed by Luminex 200 using Human cytokine/chemokine (42 molecules), Human cytokine/chemokine Panel II (23 molecules) and Human soluble cytokine receptor (14 molecules). The Median Fluorescent Intensity (MFI) was used to obtain the calculating cytokines, chemokines and soluble receptor concentrations in tears
Of the 79 cytokines, chemokines and soluble receptors, we detected 16 molecules that demonstrated a significant differences in tears from Keratoconus patients. In cytokines, G-CSF (p<0.001), IL1-ra (p<0.05), VEGF (p<0.05), IL-16 (p<0.05) and IL-20 (p<0.05) were significantly increased in Keratoconus patients compared to control subjects. MDC (p<0.05) and GRO (p<0.05) of chemokines and sIL-1RI (p<0.05) and sIL4R (p<0.001) of soluble receptors were increased in keratoconus patients. Whereas IL4 and IFN gamma (all p<0.001) and FGF2 ( p<0.05) of cytokines, MIP1a and MIP1b (all p<0.001) of chemokines, sCD30 and sIL6R (all p<0.05) were significantly decreased in keratoconus patients
In tears of keratoconus patients, nine molecules were elevated keratoconus patients, whereas 7 molecules were decreased. It is suggested that different levels of inflammatory regulated cytokines/chemokines/soluble receptors may all play an important role in the pathogenesis of keratoconus
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