June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Detection of natural helper cells in innate immune system-dependent mouse conjunctivitis model
Author Affiliations & Notes
  • Yosuke Asada
    Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
    Frontier Research Initiative, Institute of Medical Science, University of Tokyo, Tokyo, Japan
  • Akira Matsuda
    Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • Kanji Hori
    Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • Satoshi Iwamoto
    Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • Nobuyuki Ebihara
    Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • Susumu Nakae
    Frontier Research Initiative, Institute of Medical Science, University of Tokyo, Tokyo, Japan
  • Akira Murakami
    Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • Footnotes
    Commercial Relationships Yosuke Asada, None; Akira Matsuda, None; Kanji Hori, None; Satoshi Iwamoto, None; Nobuyuki Ebihara, None; Susumu Nakae, None; Akira Murakami, SEED(Japan) JP4855782 (P), SEED(Japan) JP5132958 (P)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2077. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Yosuke Asada, Akira Matsuda, Kanji Hori, Satoshi Iwamoto, Nobuyuki Ebihara, Susumu Nakae, Akira Murakami; Detection of natural helper cells in innate immune system-dependent mouse conjunctivitis model. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2077.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: Natural helper (NH) cells are identified innate lymphocyte population, which respond to a combination of interleukin (IL)-2 and IL-25 or IL-33 to produce large amounts of TH2 cytokines and induce eosinophil infiltration in the lung- and gut-associated lymphoid tissues. We identified NH cells in mouse lacrimal- and conjunctival- tissues obtained from papain-induced conjunctivitis model, in which innate immune system dependent conjunctival eosinophilic infiltration was observed.

Methods: To made papain-induced conjunctivitis model mice, papain-soaked soft contact lens (2mm diameter) was inserted into the conjunctival sacs of C57/BL6(B6) mice or B6-Rag2 knockout (KO) mice, which lack acquired immune systems, and the eye lids were sutured. 4days after contact lens installation, the eyes were subjected to histological analysis. Flow cytometric analyses were performed using anti-Siglec-F and anti-CCR3 antibodies to quantify infiltrated eosinophils. Spleen, thymus, cervical lymph node, lacrimal gland, and conjunctival tissues obtained from the papain-induced conjunctivitis model mice were subjected to cytometric analysis. At the same time, serum IgE concentration of the mice was quantified.

Results: We identified the existence of NH cells (Lineage- ST2+ CD25+CD127+Sca1+Thy1+) in the conjunctival- and lacrimal gland -tissues obtained from papain-induced conjunctival model. Papain-induced conjunctivitis model showed comparable degree of eosinophil infiltration in the conjunctivae of Rag2 KO mice compared to those of wild-type mice. (53 ± 34 versus 73 ± 44, mean number of eosinophil ± SD per slides, n = 6 , P> 0.05 by Mann-Whitney’s U test ) Siglec-F+CCR3+ eosinophils were increased in the conjunctivae of papain-induced conjunctivitis model compared to naïve conjunctivae. (0.126 versus 0.0679, the ratio of Siglec-F+CCR3+/CD45+ cells). Total IgE concentrations were unchanged during conjunctivitis induction using papain-soaked contact lens.

Conclusions: We made innate immune system-dependent conjunctivitis model with papain-soaked soft contact lenses. NH cells were identified in the conjunctival tissue and in the lacrimal tissue of the papain-induced conjunctivitis model.

Keywords: 475 conjunctivitis  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×