June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Role of IL-7 in Corneal Lymphangiogenesis
Author Affiliations & Notes
  • Tatiana Ecoiffier
    Vision Science, University of California, Berkeley, School of Optometry, Center for Eye Disease and Development, Berkeley, CA, 94720, USA, CA
  • Maria Iolyeva
    Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Zurich, Switzerland
  • Cornelia Halin
    Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Zurich, Switzerland
  • Lu Chen
    Vision Science, University of California, Berkeley, School of Optometry, Center for Eye Disease and Development, Berkeley, CA, 94720, USA, CA
  • Footnotes
    Commercial Relationships Tatiana Ecoiffier, None; Maria Iolyeva, None; Cornelia Halin, None; Lu Chen, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2088. doi:
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      Tatiana Ecoiffier, Maria Iolyeva, Cornelia Halin, Lu Chen; Role of IL-7 in Corneal Lymphangiogenesis. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2088.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Interleukin-7 (IL-7) is an immunoregulatory cytokine that have been recently identified on lymphatic endothelial cells. The purpose of this study was to investigate the in vivo effect of IL-7 on corneal lymphangiogenesis (LG, the growth of new lymphatic vessels).

Methods: Uniform slow-release IL-7 pellets were prepared by mixing IL-7 (Peprotech, Rocky Hill, NJ) or appropriate control with sucralfate and hydron (Sigma Aldrich, St. Louis, MO). A micropocket was surgically created in BALB/c mouse cornea and a pellet was implanted 1.0 mm apart from the limbal vascular arcade. Corneas were harvested after 2 weeks and stained with LYVE-1, lymphatic specific marker. Percentage LG covered area under both control and IL-7 treated conditions were evaluated using NIH Image J software.

Results: Pellets containing IL-7 induced a 6.4 fold increase in the corneal area covered by lymphatic vessels compared to the control condition (p < 0.05).

Conclusions: Our data demonstrate that IL-7 is a strong stimulator of lymphangiogenesis in vivo. This study will shed some new light on our understanding of corneal lymphangiogenesis and potentially promotes the development of novel therapeutic strategies for lymphatic related diseases occurring inside and outside the eye.

Keywords: 609 neovascularization • 480 cornea: basic science  
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