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Mirela Krasniqi, Heidi Rassavong, Rachida Bouhenni, Jeffrey Dunmire, Sami Al Shahwan, Ibrahim Al Jadaan, Hind Alkatan, Deepak Edward; Lymphatic and Blood Vessel Density in Human Conjunctiva Following Glaucoma Surgery. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2121. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the lymphatic vascular microvessel density (LVD) and the blood vascular microvessel density (MVD) and their distribution in excised leaking blebs after mitomycin C (MMC) trabeculectomy and normal conjunctiva specimens.
LVD and MVD in normal human conjunctiva (n=7) and excised blebs in the hypocellular stroma and peribleb tissue (conjunctiva adjacent to hypocellular bleb tissue, n=7) was evaluated by immunohistochemistry using antibodies raised against Lymphatic Vessel Endothelial Receptor 1 (LYVE-1, lymphatic vessels) and CD34 (blood vessels). LVD and MVD counts were performed by light microscopy in 5 fields at 20X magnification by three observers. Differences were determined using Mann Whitney U test (p<0.05 was considered significant).
The LVD and MVD were significantly reduced to absent in the hypocellular conjunctival stroma of the excised blebs compared to normal conjunctiva (21.42 vs. 1.16, p<0.002 and 24.28 vs. 1, p< 0.008 respectively). The LVD and MVD was also reduced (2 to 2.5 fold) in the peribleb stroma when compared to normal conjunctiva specimens.
Reduced LCD and MVD in the hypocellular stroma and peribleb stroma may play a role in an altered immune response in leaking blebs leading to an increased risk of blebitis.
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