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Mario Matthaei, Jianfei Hu, Laura Kallay, Claus Cursiefen, Jiang Qian, Albert Jun; MicroRNA Analysis in Fuchs Endothelial Corneal Dystrophy. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2197.
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© ARVO (1962-2015); The Authors (2016-present)
MicroRNAs (miRNAs) are a class of endogenous noncoding RNA which posttranscriptionally modulate gene expression during development and disease. Our study investigated the differential miRNA expression pattern in human Fuchs Endothelial Corneal Dystrophy (FECD) compared to normal endothelium.
Total RNA was extracted from corneal endothelial cells of FECD eyes (n=6) and age-matched normal autopsy globes (n=6). The expression of 754 well characterized miRNA sequences was analyzed in preamplified cDNA samples using OpenArray plate technology on the QuantStudio 12K Flex system for high-throughput real-time quantification and individual targets were validated by Taqman qPCR assays.
We detected differential expression of at least 37 microRNAs using global normalization and applying a fold-change of 2 and p=0.05 as cut-off values. Of these 37 miRNAs, 32 showed downregulation whereas 5 miRNAs were upregulated. MiRNA target genes were predicted and further analyzed by gene ontology assessment.
We present the first endothelial cell microRNA profile in FECD and normal endothelial samples and identify dysregulated miRNA expression in FECD.
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