June 2013
Volume 54, Issue 15
ARVO Annual Meeting Abstract  |   June 2013
Tight Junction Transmembrane Protein Claudin Subtype Expression and Distribution in Human Corneal Endothelium
Author Affiliations & Notes
  • Emi Inagaki
    Ophthalmology, Keio University, Tokyo, Japan
  • Shin Hatou
    Ophthalmology, Keio University, Tokyo, Japan
  • Satoru Yoshida
    Ophthalmology, Keio University, Tokyo, Japan
  • Hideyuki Miyashita
    Ophthalmology, Keio University, Tokyo, Japan
  • Kazuo Tsubota
    Ophthalmology, Keio University, Tokyo, Japan
  • Shigeto Shimmura
    Ophthalmology, Keio University, Tokyo, Japan
  • Footnotes
    Commercial Relationships Emi Inagaki, None; Shin Hatou, None; Satoru Yoshida, None; Hideyuki Miyashita, None; Kazuo Tsubota, AcuFocus, Inc (C), Allergan (F), Bausch Lomb Surgical (C), Functional visual acuity meter (P), JiNS (P), Kissei (F), Kowa (F), Santen, Inc. (F), Otsuka (F), Pfizer (C), Thea (C), Echo Denki (P), Nidek (F), Ophtecs (F), Wakasa Seikatsu (F), CEPT Company (P); Shigeto Shimmura, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2199. doi:
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      Emi Inagaki, Shin Hatou, Satoru Yoshida, Hideyuki Miyashita, Kazuo Tsubota, Shigeto Shimmura; Tight Junction Transmembrane Protein Claudin Subtype Expression and Distribution in Human Corneal Endothelium. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2199.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: The primary function of corneal endothelium is to maintain corneal transparency by regulating corneal hydration and nutrition modulated by barrier and metabolic pump function. However, the molecular mechanism of its barrier function is still relatively unknown. Claudins, recently identified main components of tight junctions,are a family of four-transmembrane-spanning proteins. To date, 24 subtype of claudins have been identified in human. Combination of claudin subtypes may contribute not only to the tightness of tight junction strands but also ion-selective channels. In this study, we investigated the expression and pattern of claudins in in vivo human cornea.

Methods: The experiments in this paper used remained corneal tissue supplied from USA eye bank after the central buttons were used for corneal transplantation. We stripped corneal endothelium with Descemet membrane and corneal epithelium from corneal stroma. Reverse transcription-polymerase (RT-PCR) was performed to evaluate that subtypes of claudins expressed in corneal endothelium, stroma and endothelium. Then, immunohistochemistry was performed for claudins positively expressed in RT-PCR, to confirm whether they would express lateral side of corneal endothelium.

Results: Transcripts for claudin-1, -2, -3, -4,-7, -10b, -11,-12,-14, -15, -22, -23, and -24 were identified in human corneal endothelium in RT-PCR. Claudin-1, -2, -3, -4, -7 , -11, -12, -14, -15, -22, -23, and -24 expression in corneal endothelium was common to corneal epithelium, and claudin-1, -2,-3,-4, -7, -11,-12,-14, -15, -22,-23,and -24 was common to corneal stroma. Among the claudin subtypes expressed in corneal endothelium, immunohistochemistry revealed the expression of claudin-1,-2, -4, -7, -10 antibodies showed bands that correspond to the junctional complex. Claudin-11 was also expressed in corneal endothelium, however, the expression was not continuous but in a dotlike pattern along cell junctions.

Conclusions: Claudin-1, -2, -4,-7, -10b and -11 are expressed in corneal endothelium. This combination of claudin subtype may contribute to the uniqueness of barrier integrity and ion sensitivity in corneal endothelium.

Keywords: 481 cornea: endothelium • 446 cell adhesions/cell junctions  

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