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Lan Wang, Hongli Yang, Massimo Fazio, Brandon Smith, Chad Cheetham, J Crawford Downs, Juan Reynaud, Howard Lockwood, Claude Burgoyne, Christopher Girkin; Three-Dimensional (3-D) Histomorphomety of Normal Human Optic Nerve Head (ONH) Connective Tissue at physiologic and elevated IOP. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2259.
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© ARVO (1962-2015); The Authors (2016-present)
To compare the difference of normal human ONH connective tissue structure at physiologic and elevated IOP by 3D histomorphometry.
38 eyes from 19 human donors (54±17 years old), collected within 6 hours postmortem underwent aldehyde fixation at 10mmHg IOP in both eyes (7 pairs) or 10 mmHg in the right eye and 45 mmHg in the left eye (12 pairs). The ONH and peripapillary sclera were trephinated and serial sectioned using an automated microtome-based system that yielded serial fluorescent images of the block face as we cut through the ONH at 1.5 μm section thickness; the autofluorescent images were aligned and stacked into a 3D ONH reconstruction with 1.5x1.5x1.5 μm voxel resolution (Downs et al. ISER 2010). The ONH and peripapillary scleral anatomy was delineated in custom software (Downs, Yang et al. IOVS 2007). The 80 Bruch’s membrane opening (BMO) points were used to establish a zero reference plane for all measurements. The BMO radius, neural canal depth, lamina cribrosa thickness and depth, and scleral thickness were calculated and analyzed using mixed effects models with generalized estimation equations.
In adjusted models, IOP elevation from 10 to 45 mmHg resulted in a significant decrease in the posterior scleral canal opening radius (p = 0.028) and posterior laminar insertion radius (p = 0.026). No other significant differences in morphometric parameters were detected (Table 1).
While no significant changes in laminar position were seen, contraction of the posterior neural canal occurs in response to IOP elevation in normal human donor eyes.
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