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Carmen Mendez-Hernandez, Ignacio Rodriguez-Uña, Paula Arribas Pardo, Federico Saenz-Frances, Manuel González-de-la-Rosa, Jose Martinez de la Casa, Enrique Santos-Bueso, Julian Garcia-Feijoo, PI09/90933 of the Instituto de Salud Carlos III grants. Red Temática de Investigación Cooperativa ISCIII: RD07/062; Reproducibility of the measurement of Optic Nerve Head Hemoglobin levels. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2287.
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To evaluate the intraobserver, interobserver, within and between-session reproducibility of the measurement of Optic Nerve Head (ONH) Hemoglobin levels using colour analysis by Laguna ONhE (Optic Nerve Hemoglobine) program.
Observational prospective study of 29 eyes (11 hypertensive and glaucomatous; 18 healthy eyes). Two investigators obtained two retinographies (Canon non-mydriatic retinal camera CD-DGi, Canon Inc.,Tokyo, Japan) in two testing sessions three weeks apart and analyzed the images using the Laguna ONhE (Optic Nerve Hemoglobine). The following parameters were quantified: ONH Hemoglobin concentration in its whole extent and in vertical disk diameter, disc-cup ratio and GDF (Glaucoma Discriminant Function). Agreement was illustrated using Bland-Altman plots and reproducibility was assessed comparing the intraclass correlation coefficients (ICC).
In session 1, investigator 1 found mean levels of ONH Hemoglobin of 67.94 +/- 8.70% in healthy eyes and of 57.90+/-5.36% in hypertensive and glaucomatous eyes. Correspondent values for investigator 2 were 68.27 +/- 8.52% and 57.83 +/-4.88%, respectively. ONH Hemoglobin concentration measurements, in its whole as well as in the vertical diameter, showed the highest ICCs (All above 0.9). Variability was greater for GFD (ICC>0.8) and cup-disc ratio (ICC>0.71).
The measurement of ONH Hemoglobin concentration using the Laguna ONhE program shows high reproducibility both in glaucomatous and non-glaucomatous ONHs.
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