June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Repeatability of Three-Dimensional (3D) Microstructural Parameters of the Lamina Cribrosa (LC) in Adaptive Optics Spectral-Domain Optical Coherence Tomography (AO-SDOCT)
Author Affiliations & Notes
  • Zach Nadler
    UPMC Eye Center, Eye and Ear Institute, Ophthalmology and Visual Science Research Center, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA
  • Bo Wang
    UPMC Eye Center, Eye and Ear Institute, Ophthalmology and Visual Science Research Center, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA
    Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA
  • Gadi Wollstein
    UPMC Eye Center, Eye and Ear Institute, Ophthalmology and Visual Science Research Center, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA
  • Daniel Hammer
    Center for Devices and Radiological Health, Food and Drug Administration, Silver Spring, MD
  • R. Ferguson
    Physical Sciences Inc., Andover, MA
  • Hiroshi Ishikawa
    UPMC Eye Center, Eye and Ear Institute, Ophthalmology and Visual Science Research Center, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA
    Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA
  • Richard Bilonick
    UPMC Eye Center, Eye and Ear Institute, Ophthalmology and Visual Science Research Center, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA
    Department of Biostatistics, Graduate School of Public Health, University of Pittsburgh, Boston, MA
  • Jessica Nevins
    UPMC Eye Center, Eye and Ear Institute, Ophthalmology and Visual Science Research Center, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA
  • Larry Kagemann
    UPMC Eye Center, Eye and Ear Institute, Ophthalmology and Visual Science Research Center, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA
    Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA
  • Joel Schuman
    UPMC Eye Center, Eye and Ear Institute, Ophthalmology and Visual Science Research Center, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA
    Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA
  • Footnotes
    Commercial Relationships Zach Nadler, None; Bo Wang, None; Gadi Wollstein, Allergan (C); Daniel Hammer, Physical Sciences Inc. (C), Physical Sciences Inc. (P); R. Ferguson, Physical Sciences Inc (E), Physical Sciences Inc (P); Hiroshi Ishikawa, None; Richard Bilonick, None; Jessica Nevins, None; Larry Kagemann, None; Joel Schuman, Carl Zeiss Meditec, Inc. (P)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2638. doi:
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      Zach Nadler, Bo Wang, Gadi Wollstein, Daniel Hammer, R. Ferguson, Hiroshi Ishikawa, Richard Bilonick, Jessica Nevins, Larry Kagemann, Joel Schuman; Repeatability of Three-Dimensional (3D) Microstructural Parameters of the Lamina Cribrosa (LC) in Adaptive Optics Spectral-Domain Optical Coherence Tomography (AO-SDOCT). Invest. Ophthalmol. Vis. Sci. 2013;54(15):2638.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To evaluate imprecision of 3D LC microstructural parameters derived from automated analysis of scans taken using AO-SDOCT.

 
Methods
 

A multimodal AO retinal imaging system (Physical Sciences Inc., Andover MA), which includes SDOCT and scanning laser ophthalmoscopy (SLO) imaging channels, was used in this study. The OCT channel light source is centered at 1050 nm with isotropic axial and transverse resolution of ~4μm and a scanning rate of 28kHz. AO correction is applied to both OCT and SLO channels at ~20fps. Each eye was scanned twice, using a 6°x6° window centered on the LC. The OCT channel has 1024 A-lines per B-scan and 200 B-scans per volume. The en face SLO frames were used in precise registration of OCT B-scans for a 3D reconstruction. LC pores were segmented using an automated adaptive local thresholding technique of our own design. All processing was performed using Fiji (ImageJ 1.47c, NIH, http://imagej.nih.gov/ij). Tested parameters include: pore number, average pore cross-sectional area, mean laminar beam thickness, and ratio of average pore diameter to average beam thickness. Repeatability was assessed by computing measurement imprecision.

 
Results
 

Eight healthy and 12 glaucomatous eyes were included in this study. The results for microstructural parameter averages and imprecision are summarized in the Table. For all parameters the imprecision is small in comparison to the mean values, which indicates high repeatability.

 
Conclusions
 

Global parameters quantifying microstructure of the LC, as recorded with multimodal AO-SDOCT, are highly repeatable, as indicated by the low imprecision, and may be used to further probe 3D structural differences between healthy and glaucomatous eyes.

 
 
Table - LC measurements and imprecision. Figure (a) - C-mode slice section of the LC for a glaucomatous eye, (b) - Lamina (yellow) and pores (red) segmentation. (c) - 3D structure of the LC beams where hotter colors represent thicker beams. (d) - Pore segmentation within the analyzed LC. Agreement between segmentations appears in cyan.
 
Table - LC measurements and imprecision. Figure (a) - C-mode slice section of the LC for a glaucomatous eye, (b) - Lamina (yellow) and pores (red) segmentation. (c) - 3D structure of the LC beams where hotter colors represent thicker beams. (d) - Pore segmentation within the analyzed LC. Agreement between segmentations appears in cyan.
 
Keywords: 550 imaging/image analysis: clinical • 629 optic nerve • 549 image processing  
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