June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Optogenetic Targeting of ON and OFF bipolar cells for vision restoration
Author Affiliations & Notes
  • Jun-Kyo Suh
    Center for Bionics Research, Korea Inst of Science and Technology, Seoul, Republic of Korea
  • Jin Mo Kim
    Center for Bionics Research, Korea Inst of Science and Technology, Seoul, Republic of Korea
  • Hyuk-June Moon
    Center for Bionics Research, Korea Inst of Science and Technology, Seoul, Republic of Korea
  • Hyun-Joon Shin
    Center for Bionics Research, Korea Inst of Science and Technology, Seoul, Republic of Korea
  • Eunmi Hwang
    Center for Bionics Research, Korea Inst of Science and Technology, Seoul, Republic of Korea
  • Dong Hoon Kang
    Center for Functional Connectomics, Korea Institute of Science and Technology, Seoul, Republic of Korea
  • Changjoon Lee
    Center for Functional Connectomics, Korea Institute of Science and Technology, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships Jun-Kyo Suh, None; Jin Mo Kim, KIST (E); Hyuk-June Moon, KIST (E); Hyun-Joon Shin, None; Eunmi Hwang, None; Dong Hoon Kang, None; Changjoon Lee, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2678. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Jun-Kyo Suh, Jin Mo Kim, Hyuk-June Moon, Hyun-Joon Shin, Eunmi Hwang, Dong Hoon Kang, Changjoon Lee; Optogenetic Targeting of ON and OFF bipolar cells for vision restoration. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2678.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: The loss of photoreceptor cells caused by retinal degeneration leads to partial or complete blindness such as retinitis pigmentosa (RP) and age-related macular degeneration (AMD). As a strategy to restore the function in retinal degenerative diseases, several researchers suggested the functional optogenes such as channelrhodopsin-2 (ChR2) or Halorhodopsin (NpHR), microbial-type rhodopsin, which could play a role as a light sensor and could produce action potential firings in retina. Here, to employ the complete set of retinal circuits for ON/OFF signal, ChR2 and NpHR were targeted to ON-bipolar cells and OFF-bipolar cells, respectively, by target-specific promoter (Grm6 and ChX10 promoter) and Cre-loxp system.

Methods: To transduce optogenes into the retinas, we used AAV8-Y733F mutant vectors (AAV8m) that reported the improvement of the cellular transduction. And, we applied viral cassettes, AAV8m-Grm6-ChR2-YFP-Cre and AAV8m-Chx10-loxp-NpHR-mCherry-loxp. The viral vectors were injected into subretinal space of eyes. The expression of ChR2 and NpHR was examined in whole mount retina and vertical section using confocal microscope.

Results: One month after viral injection, ChR2, green fluorescence and NpHR, red fluorescence were observed in the whole mount retina. Especially, in the retinal vertical section, ChR2 and NpHR was expressed by target-specific promoters in the layer of bipolar cells. And in the PKC-alpha-positive cells, the ON bipolar cells, ChR2 was expressed, whereas NpHR was not expressed by Cre-loxp system.

Conclusions: In this study, we have showed that ChR2 and NpHR were expressed the ON-bipolar cells and OFF-bipolar cells, respectively, using Cre-loxp system. Therefore, we suggest that our systems may realize the light-sensitive inner retinal neurons in photoreceptor-deficient retinas, thereby activating the ON and OFF pathways with opposite polarity.

Keywords: 688 retina • 435 bipolar cells  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×