June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
EFFECT OF EXPERIMENTAL DIABETIC RETINOPATHY ON THE NON-IMAGE FORMING VISUAL SYSTEM
Author Affiliations & Notes
  • Pablo Sande
    Human Biochemistry/Sch of Med, University of Buenos Aires, Buenos Aires, Argentina
  • Nuria de Zavalia
    Human Biochemistry/Sch of Med, University of Buenos Aires, Buenos Aires, Argentina
  • Nicolas Belforte
    Human Biochemistry/Sch of Med, University of Buenos Aires, Buenos Aires, Argentina
  • Damian Dorfman
    Human Biochemistry/Sch of Med, University of Buenos Aires, Buenos Aires, Argentina
  • Ruth Rosenstein
    Human Biochemistry/Sch of Med, University of Buenos Aires, Buenos Aires, Argentina
  • Diego Fernandez
    Human Biochemistry/Sch of Med, University of Buenos Aires, Buenos Aires, Argentina
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2695. doi:
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      Pablo Sande, Nuria de Zavalia, Nicolas Belforte, Damian Dorfman, Ruth Rosenstein, Diego Fernandez; EFFECT OF EXPERIMENTAL DIABETIC RETINOPATHY ON THE NON-IMAGE FORMING VISUAL SYSTEM. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2695.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: We investigated the non-image forming visual system in an advanced stage of experimental diabetes in rats.

Methods: Male Wistar rats were injected (i.p.) with vehicle or streptozotocin. A group of animals was submitted to bilateral lensectomy. Retinal (ERG), and visual pathway (visual evoked potentials) function, the number of Brn3a(+) and melanopsin(+) RGC (immunohistochemistry), and melanopsin levels (Western Blot) were examined. The pupil light reflex (PRL) (after 30-s light flash), and light-induced c-Fos expression in the suprachiasmatic nuclei (SCN) were assessed. Anterograde transport was examined after an intravitreal injection of cholera toxin β-subunit, and circadian rhythms of general locomotor activity were registered in cages equipped with infrared detectors of motion.

Results: After 15 weeks of diabetes induction, clear alterations in the visual function were observed. Concomitantly with a significant decrease in the number of Brn3a(+) RGC, no differences in the number of melanopsin(+) cells, melanopsin levels, and retinal projections to the SCN and the olivary pretectal nucleus were observed. However, a significant decrease in light induced c-Fos expression in the SCN was found. In diabetic animals, the afferent PLR and the locomotor activity pattern appear to be conserved, although a delay in the time needed to re-entrainment after a phase delay was observed. In diabetic animals, lensectomy reversed the alterations in c-Fos expression and in the locomotor activity rhythm.

Conclusions: These results indicate that the neuronal substrate of the non-image forming visual system remained largely unaffected at advanced stages of diabetes and lensectomy, a relatively easy and safe surgery, could restore circadian alterations induced by diabetes.

Keywords: 458 circadian rhythms • 499 diabetic retinopathy • 531 ganglion cells  
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