June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
The imbalance of Th17 and Treg cells in the retinopathy of diabetic rat model
Author Affiliations & Notes
  • Chang He
    Zhongshan Ophthalmic Center, Sun yat-sen University, Guangzhou, China
  • Yuying Sun
    Zhongshan Ophthalmic Center, Sun yat-sen University, Guangzhou, China
  • Xiao Hu
    Zhongshan Ophthalmic Center, Sun yat-sen University, Guangzhou, China
  • Jing Wang
    Zhongshan Ophthalmic Center, Sun yat-sen University, Guangzhou, China
  • Lu Wang
    Zhongshan Ophthalmic Center, Sun yat-sen University, Guangzhou, China
  • Yizhi Liu
    Zhongshan Ophthalmic Center, Sun yat-sen University, Guangzhou, China
  • Xialin Liu
    Zhongshan Ophthalmic Center, Sun yat-sen University, Guangzhou, China
  • Footnotes
    Commercial Relationships Chang He, None; Yuying Sun, None; Xiao Hu, None; Jing Wang, None; Lu Wang, None; Yizhi Liu, None; Xialin Liu, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2697. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Chang He, Yuying Sun, Xiao Hu, Jing Wang, Lu Wang, Yizhi Liu, Xialin Liu; The imbalance of Th17 and Treg cells in the retinopathy of diabetic rat model. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2697. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: The balance between pro-inflammatory Th17 and anti-inflammatory regulatory T cell (Treg) is recently reported to be crucial for immune homeostasis. Diabetic retinopathy is regarded as a chronic inflammatory disorder with changes in immune cell function. The purpose of this study is to investigate the profile of Th17 cells and Tregs including their key transcription factors and related cytokine secretion in vascular leakage in retinal tissue of diabetic rat models.

Methods: Sprague Dawley rats were injected intraperitoneal Streptozotocin (STZ) to induce hyperglycemia and diabetic retinopathy. Blood glucose and body weight were monitored. At three months after induction, retinal vascular permeability was measured in vivo by the Evans Blue dye method. Flow cytometry was used to evaluate the frequencies of IL-17-producing T cells and CD4+CD25+Foxp3+ Tregs in spleen and periphery blood. In addition, mRNA levels of ROR-γt and Foxp3 (transcription factors of Th17 and Treg cells, respectively) in retinas are quantified using real-time quantitative PCR. Levels of cytokines produced by Th17 and Treg cells (IL-17 and TGF-β) and VEGF are measured by real-time quantitative PCR, Western blot and immunofluorescence.

Results: At three months after STZ treatment, STZ-treated diabetic rats demonstrated significant blood-retinal barrier (BRB) leakage and increased VEGF level compared with non-diabetic control rats. Th17 cells related transcription factor (ROR-γt) was activated and expression of its associated cytokine (IL-17) increased in the diabetic retinas with respect to non-diabetic control. The proportion of IL-17 and TGF-β was significantly elevated, suggesting the imbalance between Th17 cells and Tregs may exist in the diabetic retinas. In addition, the bias of Th17/Treg was strongly correlated to VEGF level in diabetic retinas.

Conclusions: Our results indicated that a loss of immune-balance of Th17 to Treg may have a critical role in vascular leakage and further shed new light into understanding the immune-inflammatory mechanism of diabetic retinopathy.

Keywords: 499 diabetic retinopathy • 557 inflammation • 555 immunomodulation/immunoregulation  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×