Differentiating vitreous proteomes in proliferative diabetic retinopathy using high-performance liquid chromatography coupled to tandem mass spectrometry

Abstract

 

Purpose

 

The aim of this study was to compare the protein profile of vitreous humour from diabetic patients with proliferative diabetic retinopathy with that from nondiabetic subjects with idiopathic epiretinal macular membranes.

 

Methods

 

Vitreous humour from type 2 diabetic patients with PDR (n=8) and from nondiabetic subjects with idiopathic epiretinal macular membranes (n=8) were studied. The comparative proteomic analysis was performed using reversed phase high-performance liquid chromatography coupled to electrospray Ionization tandem mass spectrometry.

 

Results

 

91 significant differentially expressed proteins (abundance ratio > 1.5, p < 0.05) were identified, including 37 and 54 proteins up- and downregulated in PDR vitreous compared with the control, respectively.

 

Conclusions

 

These data provide insight into the molecular events possibly involved in the pathogenesis of PDR and widen the scope of potential avenues for new therapies for PDR.

 

 

Process used to identify and quantify proteins by LC-ESI-MS/MS. The LC-MS data from the different groups was displayed as two-dimensional signal intensity maps with retention time and m/z on the two axes and a gray scale representing the intensity of a peak at a certain retention time and m/z. The chart represents m/z (vertical) versus retention time (horizontal).

 

Keywords: 663 proteomics • 499 diabetic retinopathy • 763 vitreous